摘要
探讨血必净注射液(Xuebijing injection,XBJ)干预cGAS/STING通路减轻脓毒症诱导的急性肺损伤(acute lung injury,ALI)的作用。采用盲肠结扎与穿刺(CLP)建立小鼠脓毒症ALI模型,LPS刺激RAW264.7细胞构建细胞炎症模型。利用HE染色、ELISA法、Western blot等方法,考察XBJ对脓毒症小鼠肺组织损伤与cGAS/STING通路相关蛋白表达的影响。结果发现,在体内,XBJ干预能够缓解肺组织损伤,降低血清IL-6、TNF-α、IFN-β、IL-1β水平,肺组织cGAS、STING、p-TBK1和p-IRF3蛋白的表达。在体外,XBJ干预后能够降低RAW264.7细胞相关炎症因子mRNA水平,以及c GAS/STING通路蛋白的表达。结果表明,XBJ能够通过抑制cGAS/STING通路活化,抑制炎症反应而发挥防治脓毒症ALI的作用。本研究为临床XBJ防治脓毒症ALI提供新的分子机制。
To investigate the effect of Xuebijing injection(XBJ)on cGAS/STING pathway in alleviating sepsisinduced acute lung injury(ALI),the mouse sepsis-induced ALI model was established by cecal ligation and puncture(CLP),and the cell inflammation model was constructed by LPS stimulating RAW264.7 cells.The effects of XBJ on lung tissue injury and cGAS/STING pathway-related protein expression in septic mice were investigated by HE staining,ELISA,and Western blot.The results showed that XBJ intervention could alleviate lung tissue injury,reduce serum IL-6,TNF-α,IFN-β,IL-1-βlevels,and the expression of cGAS,STING,p-TBK1,and p-IRF3 proteins in lung tissue in vivo,and reduce the mRNA level of related inflammatory factors in RAW264.7 cells and the expression of cGAS/STING pathway proteins in vitro.The results showed that XBJ could play a role in the prevention and treatment of sepsis-induced ALI by inhibiting the inflammatory response via inhibition of the activation of cGAS/STING pathway.This study provides a new molecular mechanism for the clinical prevention and treatment of sepsis-induced acute lung injury with XBJ.
作者
秦响英
张力元
唐嘉辉
岳孟
寇俊萍
张媛媛
QIN Xiangying;ZHANG Liyuan;TANG Jiahui;YUE Meng;KOU Junping;ZHANG Yuanyuan(Department of Pharmacology of Chinese Materia Medica,School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 211198,China)
出处
《中国药科大学学报》
北大核心
2025年第3期350-357,共8页
Journal of China Pharmaceutical University
基金
国家自然科学基金项目(No.81773971)。
