摘要
同型产乙酸是CO_(2)生物利用的重要途径,但该过程会受到O_(2)组分的干扰。文章探讨不同温度对无氧条件和有氧条件下CO_(2)同型产乙酸效率、污泥产甲烷和产乙酸活性、电子传递活性和辅酶F 420活性及其微生物群落结构的影响。结果表明:在35℃有氧条件下未产生乙酸,但在15℃有氧条件下,同型产乙酸的累积乙酸产量为(174.8±0.4)mg/g;相反,在15℃有氧条件下累积甲烷产量下降了82.0%,由35℃的(198.1±9.7)mL/g下降为15℃的(35.6±9.3)mL/g。低温条件不仅能抑制电子传递活性和辅酶F 420的活性,而且可以富集同型产乙酸菌Acetobacterium。因此,在有氧条件下二氧化碳同型产乙酸过程中,控制低温不仅有助于抑制甲烷生成,而且能够提高乙酸累积产量。
Homoacetogenesis is an important pathway for CO_(2)bio-utilization,but the process is interfered by O_(2).This study investigated the effects of different temperatures on the efficiency of homoacetogenesis,methanogenic and acetic acid-producing activities,INT-ETS and coenzyme F 420 activities,and the microbiological community structure under anaerobic and aerobic conditions.The results showed that no HAc was produced under aerobic conditions at 35℃,but at 15℃,the cumulative HAc production of homoacetogenesis was(174.8±0.4)mg/g under aerobic conditions;on the contrary,the cumulative CH_(4) production decreased by 82.0%,from(198.1±9.7)mL/g at 35℃ to(35.6±9.3)mL/g at 15℃.Low-temperature conditions not only inhibited the activities of INT-ETS and coenzyme F 420,but also enriched the typical homoacetogens Acetobacterium.Therefore,the low-temperature control during homoacetogenesis under aerobic conditions not only helped to inhibit the methanogenesis,but also increased the cumulative yield of HAc.
作者
王昱伟
王伟
李永存
何江浩
WANG Yuwei;WANG Wei;LI Yongcun;HE Jianghao(School of Civil and Hydraulic Engineering,Hefei University of Technology,Hefei 230009,China)
出处
《合肥工业大学学报(自然科学版)》
北大核心
2025年第6期721-726,749,共7页
Journal of Hefei University of Technology:Natural Science
基金
国家重点研发计划资助项目(2019YFC0408502)
国家自然科学基金区域创新发展联合基金资助项目(U19A20108)。
关键词
同型产乙酸
二氧化碳
乙酸
甲烷
嗜氢产甲烷菌
homoacetogenesis
carbon dioxide
acetic acid
methane
hydrogenotrophic methanogen
