摘要
旨在探讨紫外发光二极管(UV-LED)对猪场水源中重要病原微生物的灭活效率,并分析经UV-LED消杀的饮水对断奶仔猪生长性能与免疫指标的影响。通过菌落计数法测定UV-LED对副猪格拉瑟菌(Glaesserella parasuis,GPS),猪链球菌2型(Streptococcus suis serotype 2,SS2)和肠产毒素型大肠杆菌(enterotoxigenic Escherichia coli,ETEC)3种致病菌菌株的杀灭效率;培养非洲绿猴肾细胞(Vero),非洲绿猴胚胎肾细胞(Marc-145),猪肾细胞(PK-15)与猪肺泡巨噬细胞(PAM),对其分别接种伪狂犬病病毒(PRV)、猪圆环病毒2型(PCV2)、猪繁殖与呼吸综合征病毒(PRRSV)和非洲猪瘟病毒(ASFV),观察细胞病变情况,并计算其病毒滴度,作为UV-LED灭活病毒效果的评价指标;给断奶仔猪饲喂经UV-LED消杀的饮水,测定其对生长性能及血液指标的影响。结果:UV-LED对于水源中的GPS、SS2、ETEC这3种病菌均具有很强的杀灭效果,处理17 s以上时,对以上细菌的杀灭效率可达99.90%以上;对PRV、PCV2、PRRSV、ASFV这4种病毒均具有杀灭作用,但杀灭效果存在一定区别,处理17 s以上可完全灭活PRV、PCV2与PRRSV,而完全灭活ASFV需处理58 s;断奶仔猪饲喂结果表明,给予经UV-LED消杀饮水,可显著减少断奶仔猪腹泻率(P<0.05),改善肠道功能,并且可使断奶仔猪血液中白细胞数、淋巴细胞数与单核细胞数显著下降(P<0.05)。综上,UV-LED消杀处理可以有效消杀水中常见的病原微生物,减少断奶仔猪被病原微生物感染的风险,可应用于水源消毒。
This study was to investigate the inactivation efficiency of ultraviolet light-emitting diodes(UV-LEDs)on key pathogenic microorganisms in water sources and to analyze the effect of UV-LED sterilized drinking water on the growth performance and immunity index of weaned piglets.Firstly,we calculated the killing efficiency of UV-LED against three pathogenic bacterial strains,Glaesserella parasuis(GPS),Streptococcus suis serotype 2(SS2),and enterotoxigenic Escherichia coli(ETEC),using the colony counting method.Secondly,pseudorabies virus(PRV),porcine circovirus type 2(PCV2),porcine respiratory and reproductive syndrome virus(PRRSV),and African swine fever virus(ASFV)were inoculated into Vero cells,Marc-14 cells,PK-15 cells,and PAM cells,respectively.The efficiency of UV-LED inactivation of viruses was evaluated by calculated viral titers based on cell lesions.Finally,we further analyzed the effects of growth performance and blood indexes in weaned piglets fed with UV-LED-sterilized drinking water.Our results showed that UV-LED had a remarkable killing effect on the three pathogens,GPS,SS2,and ETEC,in drinking water.The killing efficiency of the bacteria exceeded 99.90%after 17 seconds of drinking water treatment.For all the tested viruses,UV-LED was able to completely inactivate three of the four viruses after 17 seconds of the treated drinking water,whereas complete inactivation of ASFV requires 58 seconds of treatment.The results of the animal feeding experiment showed that the administration of UV-LED-sterilized drinking water significantly reduced the diarrhea rate(P<0.05),improved the intestinal function as well as significantly decreased the number of white blood cells,lymphocytes,and mononuclear cells in the blood of the weaned piglets(P<0.05).Altogether,UV-LED treatment effectively eliminated pathogens in drinking water for pigs,reduced the risk of infection in the animals,and possessed the value of being applied to the disinfection of water sources.
作者
纪春晓
刘剑英
聂祝运
王东亮
刘德权
邹忠
唐宇龙
JI Chunxiao;LIU Jianying;NIE Zhuyun;WANG Dongliang;LIU Dequan;ZOU Zhong;TANG Yulong(College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China;Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China;PUSISAT Optoelectronic Technology Co.,Ltd.,Changsha 410205,China;College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China)
出处
《畜牧与兽医》
北大核心
2025年第7期89-96,共8页
Animal Husbandry & Veterinary Medicine
基金
湖南省自然科学基金项目(2023JJ50314)
湖北省农业生态产品价值实现工程研究项目(2023-DFZD-57)
云南省重大科技专项(202102AE090046)。
关键词
紫外发光二极管(UV-LED)
猪场
水源
消毒
病原微生物
ultraviolet light-emitting diodes(UV-LED)
piggeries
water sources
sterilization
pathogenic microorganism
