摘要
用麝香酮治疗溃疡性结肠炎后,通过旷场实验、悬尾实验观察小鼠的行为学变化,探讨麝香酮对抑郁及焦虑的缓解作用,并通过步态分析评估麝香酮对溃疡性结肠炎的药理作用效果.旷场实验结果显示,麝香酮未能显著改变溃疡性结肠炎小鼠的总运动距离,没有改变旷场中心区域、边界区域和外围区域的运动距离,麝香酮也没有显著改变溃疡性结肠炎小鼠在旷场中心、边界和外围区域停留的时间.悬尾实验结果显示,麝香酮组与模型对照组小鼠的平均不动时间几乎相同.步态分析发现,麝香酮组小鼠的步伐推动时间、制动时间、跨步时间显著缩短,跨步频数及总的跨步数显著增加,跨步长度显著加长.研究结果表明麝香酮未能显著改善抑郁及焦虑症状的行为学指标,这可能与本实验的给药方式有关;麝香酮明显改变了溃疡性结肠炎小鼠的步态特征,其可能原因是麝香酮能够缓解溃疡性结肠炎小鼠的腹部疼痛,从而改善运动能力.
After the treatment of ulcerative colitis with muscone,the behavioral changes of mice were observed by open field experiment and tail suspension test,the effect of muscone on depression and anxiety was discussed,and the pharmacological effect of muscone on ulcerative colitis was evaluated by gait analysis.The results of open field experiment show that muscone could not significantly change the total movement distance of mice with ulcerative colitis,and could not change the movement distance of the central,boundary and peripheral areas of the open field,nor does muscone significantly change the stay time of mice with ulcerative colitis in the center,boundary and peripheral areas of the open field.The results of tail suspension test show that the average immobility time of mice in the muscone group is almost the same as that in the model control group.Gait analysis shows that the pushing time,braking time and stride time of mice in the muscone group are significantly shortened,stride frequency and total stride number are significantly increased,and stride length is significantly lengthened.The results show that muscone does not significantly improve behavioral indicators of depression and anxiety symptoms,which may be related to the mode of administration in this study;muscone can significantly change the gait characteristics of mice with ulcerative colitis,possibly because muscone can relieve abdominal pain in mice with ulcerative colitis,thereby improving motor ability.
作者
姚刚
赵成
赵海
张健
张伶燕
YAO Gang;ZHAO Cheng;ZHAO Hai;ZHANG Jian;ZHANG Lingyan(Institute of Laboratory Animal Science,Guizhou University of Traditional Chinese Science,Guiyang,Guizhou 550025,China;Yibin Key Laboratory of Zoological Diversity and Ecological Conservation,Yibin University,Yibin,Sichuan 644000,China)
出处
《宜宾学院学报》
2025年第6期102-108,共7页
Journal of Yibin University
基金
贵州省科技基金项目(黔科合基础﹝2020﹞1Y386)
2023年度贵州中医药大学学术新苗项目(贵科合学术新苗﹝2023﹞-55号)。
关键词
麝香酮
溃疡性结肠炎
旷场实验
悬尾实验
步态分析
muscone
ulcerative colitis
open field test
tail suspension test
gait analysis
