摘要
为全面揭示ZmSPL基因家族在玉米生长发育、生殖转变和产量构成中的潜在功能,结合基因结构、保守结构域、Mo⁃tif分布和系统进化分析,对ZmSPL家族的系统发育关系进行了深入研究.利用群体遗传学方法研究家族成员与重要农艺性状的关联性,并结合基因表达模式筛选出关键候选基因.综合进化树分析显示,ZmSPL家族可分为I类和II类,其中I类成员在幼苗叶片中表现出较高的表达量,且在群体材料中具有更丰富的遗传变异.关联分析结果表明,多个ZmSPL基因的SNP位点与不同农艺性状显著相关,其中ZmSPL25为夜间高表达基因,呈现典型的昼夜节律性,参与株高与穗位高等性状的调控,表现出“一因多效性”特征;而ZmSPL30基因的变异较少,表明其相对保守.在其SBP结构域中的SNP位点S522被鉴定为调控玉米穗行数(RN)的关键位点,并在508份玉米材料中验证了含GG基因型的材料显著增加穗行数,表明该位点对玉米产量的调控具有重要意义.
To comprehensively reveal the potential functions of the ZmSPL gene family in maize growth,development,reproductive transition,and yield formation,an in-depth analysis of the phylogenetic relationships within the ZmSPL family was studied combining gene structure,conserved domains,motif distribution and phylogenetic analysis.Population genetics methods wre used to explore the association between family members and important agronomic traits,and key candidate genes wre identified through combination of gene expression patterns.Comprehensive evolutionary tree analysis shows that the ZmSPL family can be divided into Class I and Class II,with Class I members exhibiting higher expression levels in seedling leaves and displaying more genetic variation in population materials.Association analysis results indicate that SNP loci in several ZmSPL genes are significantly associated with various agronomic traits.Notably,ZmSPL25 is a night-expressed gene with a typical circadian rhythm,and is involved in regulating traits such as plant height and ear height,exhibiting the characteristics of“one cause and pleiotropic effects”.In contrast,ZmSPL30 shows less variation,indicating that it is relatively conserved.An SNP locus(S522)within the SBP domain of ZmSPL30 is identified as a key site regulating the number of ear row(RN)in maize.Validation in 508 maize samples shows that materials with the GG genotype significantly increase the number of RN,highlighting the importance of this locus in maize yield regulation.
作者
许婷婷
朱晓湘
杨海利
廖珧伶
苟才明
黄宁
刘玲
XU Tingting;ZHU Xiaoxiang;YANG Haili;LIAO Yaoling;GOU Caiming;HUANG Ning;LIU Ling(Faculty of Agriculture,Forestry and Food Engineering,Yibin University,Yibin,Sichuan 644000,China;Yibin Academy of Agricultural Sciences,Yibin,Sichuan 644000,China)
出处
《宜宾学院学报》
2025年第6期65-72,81,共9页
Journal of Yibin University
基金
国家自然科学基金项目(32101785)
西南作物基因资源发掘与利用国家重点实验室开放课题项目(SKL-KF202320)
宜宾学院启航项目(2021QH09)
国家级大学生创新创业训练计划项目(202310641044)。
关键词
玉米
SPL基因
系统发育
关联分析
节律表达
maize
SPL genes
phylogenetic analysis
association analysis
rhythmic expression
