摘要
目的:探讨背部推法对慢性疲劳综合征(chronic fatigue syndrome,CFS)大鼠运动能力和骨骼肌线粒体功能的影响。方法:将24只雄性SD大鼠随机分为空白组8只和造模组16只。采用强迫负重游泳联合慢性应激刺激法建立CFS大鼠模型,造模21天成功建立CFS模型后,将造模组16只分为模型组8只和推法组8只。推法组在造模后给予背部推法干预,20 min/次,1次/d,连续干预14天。记录各组大鼠一般情况半定量评分、体质量、力竭游泳时间;采用网屏实验评分评估大鼠四肢抓握能力;干预结束后取各组大鼠竖脊肌组织,采用生化法检测三磷酸腺苷(adenosine triphosphate,ATP)含量,分光光度法检测竖脊肌线粒体呼吸链复合物ⅠⅣ的活性。结果:造模后,与空白组比较,模型组和推法组大鼠一般情况半定量评分较高(P<0.01),力竭游泳时间和网屏实验评分较低(P<0.01,P<0.05)。干预后,与模型组比较,推法组大鼠一般情况半定量评分较低(P<0.01),力竭游泳时间和网屏实验评分较高(P<0.05或P<0.01)。与空白组比较,模型组大鼠竖脊肌组织ATP含量较低(P<0.01),线粒体呼吸链复合物Ⅰ、Ⅱ、Ⅲ、Ⅳ的活性下降(P<0.01);经背部推法干预后,推法组大鼠竖脊肌组织线粒体呼吸链复合物Ⅰ、Ⅱ、Ⅲ、Ⅳ的活性及ATP含量均高于模型组大鼠(P<0.01或P<0.05)。结论:背部推法能够改善线粒体功能和能量代谢,提高CFS大鼠运动能力,改善疲劳状态,其机制可能与提高骨骼肌线粒体呼吸链复合物Ⅰ、Ⅱ、Ⅲ和Ⅳ活性,增加ATP含量有关。
Objective To explore the effect of back-pushing manipulation on motor ability and skele⁃tal muscle mitochondrial function in rats with chronic fatigue syndrome(CFS).Methods Twenty-four male Sprague-Dawley rats were randomly divided into a blank group of 8 and a modeling group of 16.The CFS rat model was established using forced weight-bearing swimming combined with chronic stress stimulation for 21 days.After successful modeling,the modeling group was further divided into the modeling control(MC)group and the back-pushing manipulation(BPM)group,each of 8.In the back-pushing manipulation group,all rats were given daily 20-minute back pushing for 14 consecu⁃tive days after modeling.Then,all groups were recorded semi-quantitative scores of general conditions(SQS-GC),body mass,and exhaustion swimming time(EST).Moreover,the grasping ability of limbs was assessed by using the mesh screen test(MST).After the intervention,the tissue of the erector spinae muscle was taken to detect the adenosine triphosphate(ATP)content using the biochemi⁃cal method,and the activities of mitochondrial respiratory chain complexesⅠtoⅣwere detected by using the spectrophotometric method.Results After modeling,compared with the blank group,the SQSGC of the MC group and BPM groups were higher(P<0.01),while EST was shorter and the MST score was lower(P<0.01 or P<0.05).However,after intervention,compared with the MC group,the SQS-GC of the BPM group was lower(P<0.01),while EST and the MST score was higher(P<0.01 or P<0.05).Compared with the blank group,the ATP content of the modelling group was significantly lower(P<0.01),and the activities of mitochondrial respiratory chain complexes I,II,III,and IV de⁃creased significantly(P<0.01).However,after intervention,all the above values of the BPM group in⁃creased significantly,compared with the MC group(P<0.01 or P<0.05).Conclusion The back-pushing manipulation can improve the mitochondrial function and energy metabolism,as well as the exercise ca⁃pacity,and alleviate fatigue of CFS rats,which may be related to the improvement of the activities of mitochondrial respiratory chain complexes I,II,III,and IV,and the increase of ATP content in the skeletal muscles.
作者
杨小慧
裴鑫
谭若彤
谢文彬
李铁浪
Yang Xiaohui;Pei Xin;Tan Ruotong;Xie Wenbin;Li Tielang(Hunan University of Chinese Medicine,Changsha 410208,China)
出处
《中国运动医学杂志》
北大核心
2025年第4期291-297,共7页
Chinese Journal of Sports Medicine
基金
湖南省自然科学基金项目(2023JJ30454)
湖南省教育厅科学研究重点项目(22A0273)
湖南中医药大学研究生创新项目(2023CX165)。
关键词
慢性疲劳综合征
推拿
运动能力
骨骼肌线粒体功能
三磷酸腺苷
chronic fatigue syndrome
Tuina
motility
skeletal muscle mitochondrial function
ade⁃nosine triphosphate
