摘要
建立了基于细胞膜法6种凉味剂凉感强度的测定方法。6种凉味剂经乙醇超声溶解后,使用Fluor-8钙试剂盒和高通量实时荧光检测分析系统进行测定;利用实时加样、同步信号检测,同时以100μg/mL的AITC用作TRPA-1的阳性对照,10μg/mL的L-薄荷醇用作为TRPM-8通道的阳性对照,比较两种通道模式下的相应情况,选定TRPM-8通道作为后续优选条件。在该分析条件下,6种凉味剂在0.02~900μg/mL质量浓度范围内线性关系良好,相关系数均大于0.990,6种凉味剂的S_(LOD)≤0.05μg/mL,S_(LOQ)≤0.14μg/mL。该方法灵敏、准确、可靠,能较好地对6种凉味剂的凉感强度进行准确判定与比较。该方法分析时间短、抗干扰性好、定量准确,可为含清凉口味食品或烟草的质量控制及未知凉味剂含量样品的分析提供科学依据。
tIn this study,a method for determining the cold sensation intensity of six cooling agents based on cell membrane method was established.After six kinds of cooling agents were dissolved by ethanol ultrasonic,they were determined by Fluor-8 calcium kit and Qualcomm real-time fluorescence detection and analysis system.Real-time sampling and synchronous signal detection were used.At the same time,100μg/mL AITC was used as the positive control of TRPA-1,and 10μg/mL L-menthol was used as the positive control of TRPM-8 channel.Comparing the corresponding situations in the two channel modes,TRPM-8 channel was selected as the follow-up optimization condition.Under this analytical condition,the linear relationships of the six flavor coolants were good in the concentration range of 0.02-900μg/mL,and the correlation coefficients were all greater than 0.990.The S_(LOD) and S_(LOQ) of the six favor coolants were≤0.05μg/mL and≤0.14μg/mL.This method is sensitive,accurate and reliable,and can accurately judge and compare the cooling intensity of six cooling agents.This method has the advantages of short analysis time,good anti-interference and accurate quantification,and can provide scientific basis for the quality control of food or tobacco with cool taste and the analysis of samples with unknown cooling agent content.
作者
韩敬美
肖冬
王磊
殷沛沛
刘晶
王凯
HAN Jingmei;XIAO Dong;WANG Lei;YIN Peipei;LIU Jing;WANG Kai(Technology Center of China Tobacco Yunnan Industrial Co.,Ltd.,Kunming 650231)
出处
《食品工业》
2025年第5期108-112,共5页
The Food Industry
基金
云南大观实验室项目《面向东南亚地区凉感卷烟产品开发技术研究》(YNDG202301GJ01)
云南中烟科技项目(2024YL04)。
关键词
凉味剂
TRPM-8通道
L-薄荷醇
高通量实时荧光检测
cooling agent
TRPM-8 channel
L-menthol
real-time fluorescence detection of qualcomm quantity
