摘要
目的:分析4个品种地黄块根中糖类及苷类成分“配伍”特征及其清热凉血功效,为地黄药效物质基础及质量控制研究提供依据。方法:以生长期内4个品种地黄块根为研究对象,对生长期内地黄块根中糖类及苷类成分的含量进行测定,结合主成分分析(PCA)、正交偏最小二乘判别分析(OPLS-DA)及药效成分“配伍”特征筛选差异地黄样品,采用血热出血大鼠模型分析差异地黄样品的药效差异,并寻找潜在药效成分。结果:地黄膨大期与成熟期样品的化学成分及成分配伍特征均存在差异,成熟期地黄中苯乙醇苷成分配比高于膨大期,85-5地黄质量特征区别于其他品种地黄;不同差异地黄样品均可减轻血热出血大鼠模型的症状,其中成熟期地黄样品清热凉血效果区别于膨大期样品,85-5成熟期地黄样品区别于其他地黄;地黄清热凉血功效是地黄中多种成分共同作用的结果,可能与多糖、环烯醚萜苷及苯乙醇苷高配比有关。结论:生长时期和品种影响地黄质量,地黄清热凉血功效与其各类成分含量和配比有一定关系,该研究为地黄药效物质基础及质量控制研究提供依据。
Objective:To analyze the"compatibility"relationship of sugars and glycosides and the heat-clearing and bloodcooling effect of the roots of four varieties of Rehmannia glutinosa and provide a basis for research on the pharmacodynamic material basis and quality control of R.glutinosa.Methods:The content of sugars and glycosides in the roots of four varieties of R.glutinosa was determined during the growth period.The principal component analysis(PCA),orthogonal partial least squaresdiscriminant analysis(OPLS-DA),and the"compatibility"relationship of active components were employed to screen out the differential samples.A rat model of bleeding due to blood heat was used to verify the pharmacodynamic differences and the potential active components of differential samples.Results:The content and proportion characteristics of various components in roots of the four varieties of R.glutinosa during the expansion stage and the maturity stage had obvious differences.The proportion of phenylethanoid glycosides at the maturity stage was higher than that at the expansion stage.The R.glutinosa variety 85-5 had special quality characteristics among the tested varieties.All the samples alleviated the symptoms in the rat model.The effect of clearing heat and cooling blood was different between the maturity stage and the expansion stage,as well as between 85-5 samples at the maturity stage and other samples.The effect of clearing heat and cooling blood of R.glutinosa roots was the result of the combined action of multiple components in R.glutinosa roots and might be related to the high proportions of polysaccharides,iridoid glycosides,and phenylethanoid glycosides.Conclusion:The growth stage and variety affect the quality of R.glutinosa roots.The effect of clearing heat and cooling blood of R.glutinosa roots was related to the content and proportions of various components.The study can provide a basis for the basic research on the active components and quality control of R.glutinosa.
作者
陈亚曼
崔锦鹏
张娟
刘庆普
龚海燕
雷敬卫
王丰青
谢彩侠
CHEN Yaman;CUI Jinpeng;ZHANG Juan;LIU Qingpu;GONG Haiyan;LEI Jingwei;WANG Fengqing;XIE Caixia(Henan Provincial Engineering Technology Research Center for Quality Control and Evaluation of Traditional Chinese Medicine,Henan University of Chinese Medicine,Zhengzhou 450046,China;College of Agronomy,Henan Agricultural University,Zhengzhou 450002,China)
出处
《中国实验方剂学杂志》
北大核心
2025年第12期193-201,共9页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(82073952,82373985)。
关键词
地黄
药效成分
配伍
清热凉血
关联度
Rehmannia glutinosa
active components
compatibility
clearing heat and cooling blood
relational degree
