摘要
以开花和未开花筇竹(Qiongzhuea tumidinoda)叶片为实验对象,对其进行快速叶绿素荧光检测分析,研究其光合生理特性,旨在为揭示筇竹开花后死亡机制奠定理论基础。结果表明:1)未开花筇竹的荧光强度整体显著高于开花筇竹,未开花筇竹的荧光强度值为7336.33~24872.33,开花筇竹荧光值为5991.67~10453.00。2)可变荧光强度(V_(t))曲线显示开花筇竹I、P相逐渐降低,K、J相逐渐上升,△W_(OJ)、△W_(JI)、△W_(IP)曲线呈正K峰(K-band)、正H峰(H-band)和正G峰(G-band)。3)开花筇竹原初光化学效率(F_(v)/F_(o))、最大光化学效率(F_(v)/F_(m))、光能吸收性能指数(PI_ABS)、PSⅡ向下游传递电子的能力(Ψ_(o))、电子传递量子产额(φE_(o))、单位反应中心传递电子的能量(ET_(o)/RC)都低于未开花筇竹;单位反应中心吸收的光能(ABS/RC)、单位反应中心耗散掉的能量(DI_(o)/RC)、单位反应中心捕获的用于还原Q_(A)的能量(TRo/RC)、用于热耗散的量子比率(φD_(o))高于未开花筇竹。研究表明,开花筇竹光合电子传递链中PQ库的氧化还原平衡被打破,PSⅠ的氧化还原能力降低,PSⅡ受体侧Q_(A)下游的电子传递受到抑制。研究结果可为深入解析筇竹开花过程中光合作用特性及理解竹类“开花—衰老—死亡”的生命周期模式提供参考。
Using the leaves of flowering and non-flowering Qiongzhuea tumidinoda as experimental subjects,the paper conducts rapid chlorophyll fluorescence detection and analysis to investigate their photosynthetic physiological characteristics,aiming to establish a theoretical basis for uncovering the mechanism behind the post-flowering mortality of Q.tumidinoda.The results show that:1)The fluorescence intensity of non-flowering Q.tumidinoda is significantly higher than that of flowering Q.tumidinoda.The fluorescence intensity of non-flowering Q.tumidinoda ranges from 7336.33 to 24872.33,while flowering Q.tumidinoda has the intensity in the range from 5991.67 to 10453.00;2)The variable fluorescence intensity(V_(t))curve shows that the I and P phases gradually decrease,while the K and J phases gradually increase.The curves of △W_(OJ),△W_(JI) and △W_(IP) present positive K-band,positive H-band and positive G-band;and 3)The primary photochemical efficiency F_(v)/F_(o),the maximum photochemical efficiency(F_(v)/F_(m)),the light energy absorption performance index(PI_ABS),the ability of PSⅡ to transfer electrons downstream(Ψ_(o)),the quantum yield of electron transfer(φE_(o)),and the energy of electron 55 transfer per unit reaction center(ET_(o)/RC)of flowering Q.tumidinoda are lower than those of non-flowering Q.tumidinoda;And the light energy absorbed by the unit reaction center(ABS/RC),the energy dissipated by the unit reaction center(DI_(o)/RC),the energy captured by the unit reaction center for reducing Q_(A)(TRo/RC),and the quantum ratio for heat dissipation(φD_(o))are higher than those of non-flowering Q.tumidinoda.This study shows that the redox balance of PQ Library in the photosynthetic electron transport chain of Q.tumidinoda is broken,the redox capacity of PSⅠis reduced,and the electron transport downstream of Q_(A) on the receptor side of PSⅡis inhibited.The study would provide a reference for further analyzing the photosynthetic characteristics of Q.tumidinoda during flowering and understanding the bamboo life cycle of“flowering-senescence-death”.
作者
夏富娴
郑崇兰
肖学军
沈俊华
巫登峰
刘永安
刘大章
邱琳香
Xia Fuxian;Zheng Chonglan;Xiao Xuejun;Shen Junhua;Wu Dengfeng;Liu Yongan;Liu Dazhang;Qiu Linxiang(Liangshan Academy of Forestry and Grassland,Xichang 615000,Sichuan,China)
出处
《世界竹藤通讯》
2025年第3期55-63,共9页
World Bamboo and Rattan
关键词
筇竹
开花
叶绿素荧光
光系统Ⅱ
Qiongzhuea tumidinoda
flowering
chlorophyll fluorescence
photosystemⅡ
