摘要
施用外源微量元素有助于提高部分经济作物的产量与品质,然而关于外源锌对斑兰叶光合作用及挥发性成分种类和含量的影响目前尚不可知。因此,本研究在田间设置不同锌肥喷施处理:0 kg/hm^(2)(CK)、3.2 kg/hm^(2)(Zn_(1))、4.0 kg/hm^(2)(Zn_(2))和4.8 kg/hm^(2)(Zn_(3)),监测喷施锌肥后斑兰叶光合生理指标以及挥发性成分含量的时空动态变化。结果表明:Zn_(2)处理能显著提高叶绿素含量,促进叶片生长和发育,叶片数量在处理后的各个阶段均达到最大值,但长期高浓度锌肥处理可能对叶片扩展和植株高度产生抑制作用。斑兰叶光合速率、气孔导度和蒸腾速率均随锌肥浓度的增加而上升,并且在处理后约30 d,Zn_(3)处理表现最佳,Zn_(2)处理次之。Zn_(2)和Zn_(3)处理在促进多种香气成分如2-乙酰基-1-吡咯啉(2AP)、2-十六醇、棕榈酸乙酯等的积累上效果显著,其中Zn_(2)处理的2AP、2-十六醇的含量与CK相比分别增加203%和80.89%。Zn_(1)处理在喷施后20 d时2AP含量显著提高,而Zn_(3)处理则对角鲨烯、新植二烯和叶绿醇的合成有显著促进作用。斑兰叶主要挥发性成分可能受到其农艺性状及光合生理指标的调控作用影响,其中光合速率的提升和叶片数量的增加可能对角鲨烯和新植二烯的合成具有促进作用,而胞间CO_(2)浓度的升高则可能抑制2AP和角鲨烯的累积。总之,适量锌肥处理(4.0 kg/hm^(2))能有效促进斑兰叶的光合作用,提高叶绿素含量,促进叶片生长,增加挥发性物质及特征香气成分的含量,为优化斑兰叶高质效栽培技术提供理论依据。
The application of exogenous trace elements can help improve the yield and quality of certain economic crops.However,the effects of exogenous zinc on the photosynthetic physiological characteristics and volatile compounds in Pandanus amaryllifolius Roxb.(Pandan)remain unclear.Therefore,this study established a field control experiment on Pandan leaves with different zinc spray treatments,0 kg/hm^(2)(CK),3.2 kg/hm^(2)(Zn_(1)),4.0 kg/hm^(2)(Zn_(2)),and 4.8 kg/hm^(2)(Zn_(3)).The aim was to monitor the temporal and spatial dynamics of the photosynthetic rate and volatile compounds content following zinc fertilization.The results indicated that Zn_(2) treatment significantly increased the chlorophyll con-tent,promoted the leaf growth and development,and resulted in the highest leaf count at all stages post-treatment.However,prolonged high-concentration zinc fertilization could inhibit leaf expansion and plant height.The photosyn-thetic physiology,photosynthetic rate,stomatal conductance,and transpiration rate increased with zinc concentration.Around 30 days post-treatment,Zn_(3) treatment performed the best,followed by Zn_(2) treatment.Zinc fertilization in-creased the content of volatile compounds.Zn_(2) and Zn_(3) treatments were notably effective in promoting the synthesis of several volatile compounds,such as 2-acetyl-1-pyrroline,2-hexadecanol,and ethyl palmitate.Under Zn_(2) treatment,the content of 2-acetyl-1-pyrroline and 2-hexadecanol increased by 203%and 80.89%,respectively,compared with CK.Zn_(1) treatment significantly increased 2-acetyl-1-pyrroline content at 20 days post-application,while Zn_(3) treatment sig-nificantly promoted the synthesis of squalene,neophytadiene,and phytol.Correlation analysis showed that the main volatile compounds in Pandan interacted with agronomic traits and photosynthetic physiological indicators.The increase in photosynthetic rate and leaf count promoted the synthesis of squalene and neophytadiene,whereas elevated intercel-lular CO_(2) concentration might inhibit the accumulation of 2-acetyl-1-pyrroline and squalene.This study demonstrates that appropriate zinc fertilization can effectively enhance photosynthesis,increase chlorophyll content,promote leaf growth,and boost the content of volatile compounds and characteristic volatile compounds in Pandan.The findings would provide a theoretical basis for optimizing high-efficiency cultivation techniques for Pandan and promoting the sustainable development of Pandan-related industries.
作者
闫露
张昂
鱼欢
秦晓威
邓文明
吉训志
贺书珍
宁紫薇
鲁楠
徐灵
冯倬
YAN Lu;ZHANG Ang;YU Huan;QIN Xiaowei;DENG Wenming;JI Xunzhi;HE Shuzhen;NING Ziwei;LU Nan;XU Ling;FENG Zhuo(Institute of Spice and Beverage Research,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Genetic Im-provement and Quality Regulation for Tropical Spice and Beverage Crops of Hainan Province/Key Laboratory of Genetic Re-sources Utilization of Spice and Beverage Crops,Ministry of Agriculture and Rural Affairs,Wanning,Hainan 571533,China;College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan,Hubei 430070,China;College of Tropical Crops,Yunnan Agricultural University,Pu’er,Yunnan 665099 China)
出处
《热带作物学报》
北大核心
2025年第7期1692-1701,共10页
Chinese Journal of Tropical Crops
基金
海南省自然科学基金项目(No.323RC531)
海南省重点研发计划项目(No.ZDYF2025SXLH013)。
关键词
斑兰叶
2-乙酰基-1-吡咯啉
锌肥
农艺性状
光合特性
挥发性物质
Pandanus amaryllifolius Roxb.
2-Acetyl-1-pyrroline
zinc fertilizer
agronomic traits
photosynthetic prop-erties
volatile compounds
