摘要
通过研究早孕因子单克隆抗体EPF-B6对宫颈癌HeLa细胞增殖、迁移、克隆及凋亡的影响,探讨EPF单抗对宫颈癌细胞生物学行为的抑制作用。利用RT-PCR及Western Blot检测等6种常见肿瘤细胞中EPF基因及细胞培养液中EPF蛋白表达;CCK8、细胞划痕、平板克隆分别检测不同浓度的EPF-B6单抗对HeLa细胞增殖、迁移与群体依赖性的影响;流式细胞术检测HeLa细胞凋亡与周期。结果表明,常见肿瘤细胞中均存在EPF基因,宫颈癌HeLa细胞、乳腺癌MCF-7细胞与人卵巢癌A2780细胞培养液中有EPF蛋白的表达;EPF-B6单抗能显著抑制HeLa细胞的增殖、迁移、克隆形成能力,呈现浓度依赖性(P<0.01)。细胞周期结果显示,EPF-B6单抗将HeLa细胞的DNA复制进程阻滞在G2/M期。
The inhibitory effect of monoclonal antibody against early pregnancy factor(EPF-B6)on the biological behavior of cervical cancer HeLa cells was investigated by studying the effects of EPF-B6 on the proliferation,migration,cloning and apoptosis of HeLa cells.RT-PCR and Western Blot were used to detect the expression of EPF gene in six common cancer cells and EPF protein in cell culture medium.The effects of different concentrations of EPF-B6 monoclonal antibody on the proliferation,migration and population dependence of HeLa cells were detected by CCK8 assay,cell scratch assay and plate cloning assay,respectively.The apoptosis and cycle of HeLa cells were detected by flow cytometry.Results showed that EPF gene was present in common tumor cells.EPF protein was expressed in the culture medium of cervical cancer HeLa cells,breast cancer MCF-7 cells and ovarian cancer A2780 cells.The results showed that EPF-B6 monoclonal antibody could significantly inhibit the proliferation,migration and colony formation of HeLa cells,it was concentration-dependent(P<0.01).The results of cell cycle analysis showed that EPF-B6 monoclonal antibody blocked the DNA replication process of HeLa cells at G2/M phase.
作者
段芙春
任洪林
胡盼
李岩松
柳溪林
王菡
刘蒙迪
李浩菘
卢士英
DUAN Fu-chun;REN Hong-lin;HU Pan;LI Yan-song;LIU Xi-lin;WANG Han;LIU Meng-di;LI Hao-song;LU Shi-ying(State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases/Key Laboratory of Zoonosis Research,Ministry of Education/Institute of Zoonosis/College of Veterinary Medicine,Jilin University,Changchun 130062,China)
出处
《湖北农业科学》
2025年第5期117-122,140,共7页
Hubei Agricultural Sciences
基金
国家自然科学基金面上项目(32072943)。
关键词
早孕因子
单克隆抗体
宫颈癌
HELA细胞
增殖
迁移
凋亡
early pregnancy factors
monoclonal antibody
cervical cancer
Hela cells
proliferation
migration
apoptosis
