摘要
为探究积雪草[Centella asiatica(L.)Urban]根、茎、叶中内生真菌的分布规律,分析广西区内的积雪草内生真菌类群组成。从广西柳州市、钦州市、北海市、桂林市、贺州市、百色市6个地区采集健康的积雪草,对根、茎、叶进行内生真菌的分离。通过形态学与分子生物学相结合的方法对内生真菌进行鉴定,并开展多样性研究分析。结果表明,共分离出114株内生真菌,分别隶属于1门3纲9目12科17属,其中1个属的分类地位未定。炭疽菌属(Colletotrichum)、间座壳属(Diaporthe)为积雪草内生真菌的优势属。不同地区的积雪草其内生真菌多样性存在较大差异。桂林市、柳州市的积雪草内生真菌最为丰富,北海市与钦州市的积雪草内生真菌相似性最高,其次是桂林市与柳州市。经比较发现,根部的内生真菌丰富度最高,茎与叶的内生真菌相似性系数最高。表明广西积雪草内生真菌多样性丰富,分布较均匀,且生长环境和组织部位对其内生真菌的组成分布和多样性均有影响。
In order to investigate the distribution of endophytic fungi in the roots,stems and leaves of Centella asiatica(L.)Urban,the composition of endophytic fungi groups of Centella asiatica in Guangxi was analyzed.Healthy Centella asiatica specimens were collected from six distinct regions in Guangxi,including Liuzhou,Qinzhou,Beihai,Guilin,Hezhou,and Baise cities,followed by endophytic fungal isolation from their root,stem,and leaf tissues.The endophytic fungi were identified by combining morphological and molecular biological methods,followed by diversity analysis.The results showed that a total of 114 endophytic fungal strains were isolated,belonging to 1 phylum,3 classes,9 orders,12 families,and 17 genera,with the taxonomic status of 1 genus remaining undetermined.The genera Colletotrichum and Diaporthe were identified as the dominant endophytic fungi in Centella asiatica.The endophytic fungi diversity of Centella asiatica in different regions was significantly different.The endophytic fungi in Guilin City and Liuzhou City were the most abundant,and the endophytic fungi in Beihai City and Qinzhou City were the most similar,followed by Guilin City and Liuzhou City.Through comparative analysis,it was found that the endophytic fungi in roots had the highest abundance,and the similarity coefficient of endophytic fungi in stems and leaves was the highest.The findings indicated that Centella asiatica in Guangxi exhibited high diversity and relatively uniform distribution of endophytic fungi,with both growth environment and plant tissue type significantly influencing their community composition and diversity.
作者
梁露
梁文静
宋利沙
万凌云
谭桂玉
张占江
潘丽梅
张坤
涂冬萍
韦树根
LIANG Lu;LIANG Wen-jing;SONG Li-sha;WAN Ling-yun;TAN Gui-yu;ZHANG Zhan-jiang;PAN Li-mei;ZHANG Kun;TU Dong-ping;WEI Shu-gen(Faculty of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Botanical Garden of Medicinal Plants/Guangxi Key Laboratory ofHigh-Quality Formation and Utilization of Dao-Di Herbs,Nanning 530023,China)
出处
《湖北农业科学》
2025年第5期90-101,共12页
Hubei Agricultural Sciences
基金
广西自然科学基金项目(2023GXNSFAA026330)
广西科技基地和人才专项(桂科AD22035026)
广西中药材品质创新研究团队项目(GZKJ2305)
广西中医药适宜技术开发与推广项目(GZSY23-02)
广西岐黄学者培养项目(GXQH202402)
广西中医药管理局横向项目(GZKJ2314-4)
广西药用植物园科研基金项目(桂药基202008)。
