摘要
为明确驱动亚热带森林土壤细菌群落演替的关键环境因子,以地处长江上游的重庆市铁山坪林场马尾松针叶林、香樟阔叶林及马尾松-香樟混交林3种林型土壤为研究对象,测定其土壤基本性质,并用Illumina MiSeq测序解析了其细菌群落。结果表明,3种林型中,针叶林土壤的速效铁含量及纤维素酶活性最高。阔叶林土壤中富集了玫瑰弯菌属、链霉菌属、苔藓杆菌属、独活假丝酵母菌属和细链孢菌属等5种菌属;针叶林土壤中酸杆菌属的相对丰度稍高;芽孢杆菌属和节杆菌属则是混交林土壤的标志菌属。针叶林与阔叶林、混交林两林地的土壤细菌群落结构差异显著(P<0.05),土壤的pH值及全磷、速效磷、速效铁含量是影响该区域林地土壤细菌群落的关键因子,而纤维素酶活性亦与土壤细菌群落结构极显著相关(P<0.01)。林型是决定亚热带森林土壤细菌群落结构及其生态功能的关键因子,可为全面揭示亚热带森林土壤微生物的生态学特征及功能打下基础。
The aim of this study is to identify the key factors driving soil bacterial community composition in subtropical forests.The basic properties of the soil samples collected from three different types of forests Pinus massoniana(conifer),Cinnamomum camphora(broadleaf),and mixed P.massoniana and C.camphora in the Tieshanping Forest Farm of Chongqing were determined.The bacterial community structures of those samples were explored by Illumina MiSeq sequencing.The results indicated that among these three forest types,the conifer forest had the highest available iron content and the highest cellulase activity in soil.Five bacterial genera including Roseiarcus,Streptomyces,Bryobacter,Candidatus_Soilbacter,and Catenulispora were enriched in the broadleaf forest soil.The conifer forest soil showed higher relative abundance of Acidibacter,and Bacillus and Arthrobacter were the marker genera in the mixed forest soil.The soil bacterial community structure of the conifer forest was different from those of the broadleaf and mixed forests(P<0.05).Soil pH,total phosphorus,available phosphorus,and available iron were the key factors affecting the soil bacterial community structure in this forest region.Additionally,the cellulase activity extremely correlated with the soil bacterial community structure(P<0.01).In summary,forest type is an essential factor determining the structure and potential functions of the soil bacterial community structures in subtropical forests.The findings are essential for deeply understanding the ecological characteristics and functions of soil microorganisms in subtropical forests.
作者
李月柔
陈展
王明霞
周志峰
LI Yuerou;CHEN Zhan;WANG Mingxia;ZHOU Zhifeng(College of Resources and Environment,Southwest University,Chongqing 400715,China;Ecology and Nature Conservation Institute,Chinese Academy of Forestry,Beijing 100091,China)
出处
《森林与环境学报》
北大核心
2025年第4期368-374,共7页
Journal of Forest and Environment
基金
国家自然科学基金项目“亚热带酸雨区不同林型凋落物对土壤酸化的缓冲机理研究”(31971630)
重庆市自然科学基金项目“重庆土壤促生抑菌型铁载体菌的筛选及其微生物态效应研究”(CSTB2022NSCQ-MSX0870)。
关键词
亚热带森林
林型
土壤性质
纤维素酶活性
细菌群落结构
subtropical forest
forest type
soil properties
cellulase activity
bacterial community structure
