摘要
为明晰石质山地不同植被恢复方式对土壤酶化学计量特征的影响,探究人工林土壤微生物资源限制状况,以徐州赵疃林场石质山地2012年恢复的栾树、青桐、黄连木、五角枫人工林为研究对象,测定了0~10、10~20 cm土层的β-葡萄糖苷酶(BG)、亮氨酸氨基肽酶(LAP)、N-乙酰-β-D-葡萄糖苷酶(NAG)、碱性磷酸酶(ALP)活性以及土壤性质、细根特征等环境因子,计算了酶化学计量比,并对酶化学计量比与环境因子进行了冗余分析。结果表明:在不同植被恢复方式下,BG活性存在显著差异(P<0.05),NAG、LAP、ALP活性存在极显著差异(P<0.01)。BG、ALP活性在两土层均为黄连木人工林显著高于栾树人工林(P<0.05),NAG、LAP活性在0~10 cm土层均为栾树人工林显著高于其他人工林(P<0.05)。在不同植被恢复方式下,酶化学计量比均存在极显著差异(P<0.01),在两土层中,碳氮酶活性比均为栾树人工林最小,氮磷酶活性比均为栾树人工林最大,碳磷酶活性比在0~10 cm土层中为五角枫人工林最小,10~20 cm土层则为青桐人工林最小;不同土层间仅碳磷酶活性比未存在显著差异。冗余分析表明,土壤全氮含量、土壤碳磷比、细根全磷含量、细根全碳含量等是酶化学计量比变化的主要驱动因子。栾树人工林土壤全氮含量较低、速效磷含量较高,导致土壤微生物主要受氮限制;青桐、黄连木、五角枫人工林较高的土壤全氮含量及较低的细根全磷含量使得微生物主要受碳、磷限制。
To provide a scientific basis for soil nutrient management in rocky mountainous areas,this study investigated the effects of different vegetation restoration methods on soil enzyme stoichiometric characteristics and explored the limitations of soil microbial resources in rocky mountain plantations.The activities ofβ-glucosidase(BG),leucine aminopeptidase(LAP),N-acetyl-β-D-glucosidase(NAG),and alkaline phosphatase(ALP)in the 0-10 cm and 10-20 cm soil layers,along with soil properties and fine root characteristics of four plantations(Koelreuteria paniculata,Firmiana platanifolia,Pistacia chinensis,and Acer pictum)established in 2012 in a rocky mountainous area,were measured.Enzyme stoichiometric ratios of carbon to nitrogen,carbon to phosphorus,and nitrogen to phosphorus were calculated,and the effects of soil properties,fine root biomass and chemical composition on enzyme stoichiometric ratios were analyzed.The results showed that the activities of BG(P<0.05),NAG(P<0.01),LAP(P<0.01),and ALP(P<0.01)exerted differences among different vegetation restoration methods.In both soil layers,BG and ALP activities were significantly higher in the P.chinensis plantation than in the K.paniculata plantation(P<0.05).In the 0-10 cm soil layer,NAG and LAP activities were significantly higher in the K.paniculata plantation than in other plantations(P<0.05).Differences existed in the enzyme stoichiometric ratios among different vegetation restoration methods(P<0.01).In both soil layers,the carbon-to-nitrogen extracellular enzyme activity ratio was the smallest in the K.paniculata plantation,and the nitrogen-to-phosphorus extracellular enzyme activity ratio was the largest in the K.paniculata plantation.The carbon-to-phosphorus extracellular enzyme activity ratio in the 0-10 cm soil layer was the smallest in the A.pictum plantations and that in the 10-20 cm soil layer was the smallest in the F.platanifolia plantation.Only the carbon-to-phosphorus extracellular enzyme activity ratio did not show differences between soil layers.The redundancy analysis indicated that soil total nitrogen content,soil carbon-to-phosphorus ratio,total phosphorus content in fine roots,and total carbon content in fine roots were the main driving factors for the changes in enzyme stoichiometric ratios.In conclusion,the low total nitrogen content and high availability of phosphorus in the K.paniculata plantation led to a strong nitrogen limitation for soil microorganisms.The high available nitrogen content in soil and low total phosphorus content in fine roots of F.platanifolia,P.chinensis,and A.pictum plantations caused microbial limitations by carbon and phosphorus.
作者
张琳婧
张宇恬
史珑燕
孟董悦
徐天懂
关庆伟
ZHANG Linjing;ZHANG Yutian;SHI Longyan;MENG Dongyue;XU Tiandong;GUAN Qingwei(Co-innovation Centre for Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing,Jiangsu 210037,China;College of Ecology and Environment,Nanjing Forestry University,Nanjing,Jiangsu 210037,China)
出处
《森林与环境学报》
北大核心
2025年第4期347-357,共11页
Journal of Forest and Environment
基金
农业农村领域重大关键技术攻关项目“江苏典型困难立地高固碳树种筛选及碳增汇栽培关键技术研究与示范”(BE2022420)。
关键词
酶化学计量比
微生物资源限制
石质山地
植被恢复
酶活性
enzyme stoichiometric ratio
microbial resource limitation
rocky mountainous area
vegetation restoration
enzyme activity
