摘要
试验在盆栽条件下,以硝普钠为NO供体,设置无Na_(2)CO_(3)和NO处理(CK)、Na_(2)CO_(3)胁迫处理(T1)、Na_(2)CO_(3)+0.2 mmol/L硝普钠(T2)、Na_(2)CO_(3)+0.5 mmol/L硝普钠(T3)、Na_(2)CO_(3)+0.8 mmol/L硝普钠(T4)共5个处理,研究外源NO对Na_(2)CO_(3)胁迫下馒头柳生长、抗氧化酶活性及氮代谢相关酶活性的影响规律。结果表明,Na_(2)CO_(3)胁迫降低了馒头柳扦插苗的株高和地径,提高了其SOD活性、POD活性和MDA含量。外源喷施NO能够显著促进Na_(2)CO_(3)胁迫下馒头柳的生长,提高其SOD活性、POD活性,降低其MDA含量,缓解Na_(2)CO_(3)胁迫对馒头柳的危害。同时,外源喷施NO能够显著提高馒头柳的硝酸还原酶、谷氨酸合成酶活性,促进氨基酸合成,提高植物的氮代谢能力。综合比较来看,外源喷施NO浓度为0.5 mmol/L时缓解馒头柳Na_(2)CO_(3)胁迫的效果较好。
The experiment was conducted under pot culture conditions with sodium nitroprusside as the NO donor,and 5 treatments including no Na_(2)CO_(3)and NO treatment(CK),Na_(2)CO_(3)stress treatment(T1),Na_(2)CO_(3)+0.2mmol/L sodium nitroprusside(T2),Na_(2)CO_(3)+0.5mmol/L sodium nitroprusside(T3),Na_(2)CO_(3)+0.8mmol/L sodium nitroprusside(T4)were set up.The effects of exogenous NO on the growth,antioxidant enzyme activities and nitrogen metabolism-elated enzyme activities of Salix matsudana under Na_(2)CO_(3)stress were investigated.The results showed that Na_(2)CO_(3)stress decreased the plant height and diameter of Salix matsudana cuttings,and increased their SOD activity,POD activity and MDA content.Exogenous spraying of NO could significantly promote the growth of Salix matsudana under Na_(2)CO_(3)stress,increase its SOD activity and POD activity,reduce its MDA content and alleviate the harm of Na_(2)CO_(3)stress on Salix matsudana.At the same time,exogenous spraying of NO could significantly improve the nitrate reductase and glutamate synthase activities of Salix matsudana,promote amino acid synthesis and improve the nitrogen metabolism of plants.In a comprehensive comparison,the exogenous spraying of NO at a concentration of 0.5 mmol/L was more effective in alleviating Na_(2)CO_(3)stress in Salix matsudana.
作者
文水龙
Wen Shuilong(Guangshang Forest Farm,National Forest Administration Bureau of Lvliang Mountain in Shan xi Province,Lvliang 033404,China)
出处
《山西林业科技》
2025年第1期19-21,38,共4页
Shanxi Forestry Science and Technology
关键词
馒头柳
Na_(2)CO_(3)胁迫
生长
生理特性
Salix matsudana f.umbracalifera Rohd.
Na_(2)CO_(3)stress
Growth
Physiological property
