摘要
绞股蓝属植物资源丰富,在我国分布广泛。绞股蓝属植物因含有丰富的达玛烷型三萜皂苷且药理活性多样而引起国内外广泛关注。目前对于绞股蓝属植物化学成分及药理活性研究主要集中于绞股蓝[Gynostemma pentaphyllum(Thunb.)Makino]和长梗绞股蓝(G.longipes C.Y.Wu ex C.Y.Wu&S.K.Chen)2个种,对绞股蓝属其他种植物的研究则较少。本文采用超高效液相色谱-四极杆-飞行时间质谱联用技术,系统阐明缅甸绞股蓝(G.burmanicum King ex Chakrav)中的化学成分。首先对不同来源缅甸绞股蓝进行液质分析,观察成分组成的一致性;根据绞股蓝属来源达玛烷型三萜皂苷质谱裂解规律、丙二酰基及乙酰基皂苷成分裂解特点,结合自建数据库及ChemSipder、SciFinder、PubChem在线数据库,对表征的各个峰的结构进行鉴定;通过液质基峰离子(base peak ion,BPI)色谱图对比缅甸绞股蓝与长梗绞股蓝的成分异同。实验结果显示,不同来源缅甸绞股蓝样品的成分组成一致性良好,共从缅甸绞股蓝样品中鉴定化学成分47个,包括12个黄酮和35个三萜皂苷类成分,其中6个为新化合物。缅甸绞股蓝中的皂苷普遍存在丙二酰基和乙酰基化现象,反相C18色谱分析时它们常常在相应的原型皂苷后相继出现。色谱图显示缅甸绞股蓝与长梗绞股蓝皂苷成分一致性良好,且主要成分绞股蓝皂苷XLIX和绞股蓝皂苷A及其丙二酰基成分在2个种间含量相当。综上,通过缅甸绞股蓝的液质分析,明确了缅甸绞股蓝的成分组成及特点,为缅甸绞股蓝的开发利用提供了实验依据。
The genus Gynostemma,with abundant plant resources,is widely distributed in China.Gynostemma plants have gathered widespread attention both domestically and internationally due to their abundant contents of diverse dammarane triterpenoid saponins and various promising pharmacological activities.At present,the studies on the chemical constituents and pharmacological activities of Gynostemma plants mainly focus on G.pentaphyllum(Thunb.)Makino and G.longipes C.Y.Wu ex C.Y.Wu&S.K.Chen,with less attention given to other species within genus.In this study,the chemical constituents of G.burmanicum King ex Chakrav were systematically identified by ultra-high performance liquid chromatography-quadrupole-time of flight-mass spectrometry(UHPLCQ-TOF-MS).Firstly,the LC-MS analysis of G.burmanicum from different sources was carried out to evaluate the consistency.According to the mass spectrometry fragmentation pattern of dammarane triterpenoid saponins from Gynostemma,the fragmentation characteristics of malonylated and acetylated saponins,combined with the selfbuilt database and online database such as ChemSipder,SciFinder,PubChem,the chemical components of G.burmanicum were identified.The similarities and differences in the components between G.burmanicum and G.longipes were further assessed by comparing their base peak chromatogram.The experimental results indicated a good consistency in the composition of G.burmanicum samples from different sources.A total of 47 chemical components were identified from G.burmanicum,including 12 flavonoids and 35 triterpenoid saponins,among which 6 were new compounds.Saponins in G.burmanicum generally exhibited malonylation and acetylation,appearing after the corresponding prototypical saponins on a reversed phase chromatography.The base peak ion(BPI)chromatograms showed that the saponins of G.burmanicum were highly consistent with those of G.longipes,with comparable contents of the main components gypenoside XLIX and gypenoside A and their malonylated derivatives.In summary,this study comprehensively clarified the chemical composition and characteristics of G.burmanicum,which provided an experimental basis for the development and utilization of G.burmanicum.
作者
王美燕
李港
黄长亮
孙晴
南易
于德鑫
王秀
郭宝林
梁海珍
马百平
WANG Mei-yan;LI Gang;HUANG Chang-liang;SUN Qing;NAN Yi;YU De-xin;WANG Xiu;GUO Bao-lin;LIANG Hai-zhen;MA Bai-ping(Guangdong Pharmaceutical University,Guangzhou 510006,China;Academy of Military Medical Sciences,Academy of Military Sciences,Beijing 100850,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China)
出处
《药学学报》
北大核心
2025年第5期1499-1509,共11页
Acta Pharmaceutica Sinica
基金
国家自然科学基金面上项目(82074008)
国家自然科学基金青年项目(82404461)。
