摘要
纳米硒作为一种具有优异生物活性的元素形式,在食品、医药及农业领域具有广泛的应用价值。相比于物理、化学合成法,通过微生物合成纳米硒是一种绿色环保的方法。该研究以牛粪样品为分离源,通过梯度稀释和平板涂布法分离获得一株具有将亚硒酸钠还原为纳米硒能力的菌株。通过形态观察、16S rDNA基因测定,鉴定该菌为甘达文节杆菌(Arthrobacter gandavensis)。通过耐硒能力测定发现,该菌株最高能在含有128 mmol/L亚硒酸钠的培养基中生长,并产红色的纳米硒,其中在2 mmol/L的条件下,亚硒酸钠还原率达到86.5%。通过扫描电子显微镜发现其合成纳米硒的粒径为200~400 nm,形貌较均一。研究结果为纳米硒的生物合成提供了新的菌种资源及技术支持,同时也为功能性食品开发提供了新型硒转化菌株。
Nano-selenium,as a form of selenium with excellent biological activity,has significant application potential in the fields of food,medicine,and agriculture.Compared with physical and chemical synthesis methods,microbial synthesis of nano-selenium is an environmentally friendly approach.In this study,cow dung samples were used as the isolation source,and a strain capable of reducing sodium selenite to nano-selenium was obtained through gradient dilution and plate spreading methods.Morphological observation and 16S rDNA gene sequencing identified the strain as Arthrobacter gandavensis.Selenium tolerance tests revealed that the strain could grow in a medium containing up to 128 mmol/L sodium selenite and produce red nano-selenium.Under the condition of 2 mmol/L sodium selenite,the reduction rate reached 86.5%.Scanning electron microscopy showed that the synthesized nano-selenium particles were 200–400 nm in size with relatively uniform morphology.This study provides a new microbial resource and technical support for the biosynthesis of nano-selenium,as well as a novel selenium-converting strain for the development of functional foods.
作者
庞振斌
范诗悦
田俊龙
高超
王璋倩
程稳
何毅
董星星
PANG Zhenbin;FAN Shiyue;TIAN Junlong;GAO Chao;WANG Zhangqian;CHENG Wen;HE Yi;DONG Xingxing(National R&D Center for Se-Rich Agricultural Products Processing,Hubei Engineering Research Center for Deep Processing of Green Se-Rich Agricultural Products,School of Modern Industry for Selenium Science and Engineering,Wuhan Polytechnic University,Wuhan 430023,China;Shenzhen Hengfeng Wanda Pharmaceutical Technology Co.,Ltd.,Shenzhen 518109,China)
出处
《食品科技》
北大核心
2025年第4期1-7,共7页
Food Science and Technology
基金
武汉轻工大学校企合作技术研发项目(whpu-2024-kj-4701,Se1-202103)。
关键词
甘达文节杆菌
纳米硒
分离鉴定
Arthrobacter gandavensis
nano-selenium
isolation and identification
