摘要
糖尿病骨缺损患者伤口微环境高葡萄糖、低pH的特点使其极易受到病原菌侵害,导致骨细胞功能障碍和诱导破骨细胞形成,从而严重影响植入手术的修复效果。目前利用骨植入体修复材料聚醚醚酮(PEEK)填充缺损部位虽然已经成为一种有前景的治疗手段,却具有抗菌能力差、促成骨性能差和无法响应糖尿病微环境的缺点。为解决上述问题,本工作在PEEK表面研究构筑了一种由铜(Cu)螯合聚多巴胺(PDA)的金属多酚网络(Cu/MPN)和葡萄糖氧化酶(GOx)组成的葡萄糖响应涂层,用于增强糖尿病骨缺损的治疗效果。对修饰后的植入体表面形貌、化学组成、Zeta电位和水接触角等指标进行分析并测定了GOx负载量和近红外光(NIR)照射下的升温曲线,随后进一步验证其体外产生H_(2)O_(2)和·OH的能力,最后进行了抗菌和生物安全性检测。结果表明,该PEEK植入体成功构建Cu/MPN@GOx涂层;该涂层在葡萄糖环境下可以产生H_(2)O_(2)并进一步反应产生·OH,在高糖环境下对S.aureus和E.coli有显著抗菌作用;同时该涂层对小鼠胚胎成骨细胞(MC3T3-E1)未表现出细胞毒性,符合生物安全标准。总之,本工作提出的葡萄糖诱导抗菌和促成骨涂层修饰的新策略可为治疗顽固性糖尿病骨缺损奠定实验基础。
The characteristics of high glucose concentration and low pH value in the wound microenvironment of diabetic patients make them vulnerable to the invasion of pathogens, dysfunction of bone cells and the induction of osteoclasts, which extremely affects the repair efficacy of implantation surgery. Presently, polyetheretherketone (PEEK) has become a promising bone implant. But its poor antibacterial ability, poor osteogenic capability and unable to respond to the microenvironment of diabetes restrictes the further application. To reverse such defects, a glucose response coating composed of copper (Cu) chelated polydopamine (PDA) metal polyphenol network (Cu/MPN) and glucose oxidase (GOx) was constructed on the surface of PEEK. After analyzing the surface morphology, chemical composition, Zeta potential and water contact angle of the modified implants, the loading capacities of GOx, temperature curves under NIR and abilities to produce H_(2)O_(2) and ·OH in vitro were further verified. Finally, antibacterial and biosafety tests were conducted. The results indicated that we successfully constructed Cu/MPN@GOx coating on the PEEK surface, and it can produce H_(2)O_(2) and further react to ·OH when glucose exists. The coating had significant antibacterial effects on S.aureus and E.coli in high sugar microenvironment and it did not exhibit cytotoxicity. In conclusion, the new strategy of glucose sensitive antibacterial and osteogenic coating modification proposed in this study has laid an experimental foundation for the treatment of intractable diabetic bone defects.
作者
谢克难
何雨家
张琦
谢璐
何汶轩
XIE Kenan;HE Yujia;ZHANG Qi;XIE Lu;HE Wenxuan(School of Liquor-Brewing Engineering,Sichuan University Jinjiang College,Meishan 620860,Sichuan,China;Shijiazhuang Medical College,Shijiazhuang 050500,China;Jiannanchun Distillery Co.,Ltd.,Mianzhu 618200,Sichuan,China;Department of Restoration,West China School/Hospital of Stomatology,Sichuan University,Chengdu 610041,China;School of Chemical Engineering,Sichuan University,Chengdu 610065,China)
出处
《材料导报》
北大核心
2025年第12期230-236,共7页
Materials Reports
基金
国家自然科学基金委员会青年科学基金项目(52002255)。
