摘要
目的探讨血液透析循环管路材料的生物相容性,重点评估其与人脐静脉内皮细胞(HUVECs)的相互作用。方法通过CCK8实验和活死细胞染色,观察不同部位透析管路材料对HUVECs的黏附、生长及细胞毒性反应。结果将HUVECs在不同管路材料中进行培养增殖,不同组别的细胞在24 h后均能很好贴壁;48 h后增殖率均可达94%以上;培养至72 h后,在明场显微镜下可观察到较高细胞黏附率;6种不同的管路材料对HUVECs的黏附率都达到90%以上,且管路材料对细胞的增殖无明显影响。6组实验组分别与阴性对照组作对比,实验组与对照组差异无统计学意义(P>0.05),表明实验组细胞的增殖率、黏附率与对照组一致。从CCK8实验可得出,不同组别的细胞活力约为100%;由活死细胞感染结果可知,细胞存活率超过90%,无细胞毒性,表明具有良好的生物相容性。结论本研究能够为血液透析管路材料的优化和新型材料的开发提供科学依据,为提高透析治疗效果、减少并发症提供理论支持。
Objective To explore the biocompatibility of hemodialysis circulation pipeline materials,with a focus on evaluating their interaction with human umbilical vein endothelial cells(HUVECs).Methods Through the CCK8 experiment and staining of live and dead cells,the adhesion,growth and cytotoxic reactions of dialysis tubing materials in different parts to HUVECs were observed.Results HUVECs were cultured and proliferated in different pipeline materials,and cells in different groups could adhere well after 24 h,the proliferation rate could all reach more than 94%after 48 h.After culturing for 72 h,a high cell adhesion rate could be observed under a bright-field microscope.The adhesion rates of the six different pipeline materials to HUVECs all reached more than 90%,and the pipeline materials had no significant effect on the proliferation of cells.The six experimental groups were respectively compared with the negative control group.There was no statistically difference between the experimental group and the control group(P>0.05),indicating that the proliferation rate and adhesion rate of cells in the experimental group were consistent with those in the control group.It can be known from the staining results of live and dead cells that the cell survival rate exceeds 90%,with no cytotoxicity,indicating good biocompatibility.Conclusion This study can provide a scientific basis for the optimization of hemodialysis pipeline materials and the development of new materials,and offer theoretical support for improving the therapeutic effect of dialysis and reducing complications.
作者
周联增
郭必那
ZHOU Lianzeng;GUO Bina(Department of Equipment,Wenzhou Central Hospital,Wenzhou Zhejiang 325000,China)
出处
《中国医疗设备》
2025年第6期49-54,共6页
China Medical Devices
