摘要
目的探讨ATP生物荧光法在食堂各类餐饮具表面、工作人员手卫生监督中的应用。方法2023年1—6月使用ATP生物荧光法对某三甲医院食堂内283份消毒后的各类餐饮器具表面、工作人员手的清洁状况进行检测,并与“金标法”大肠菌群快检纸片法进行特异性、灵敏度和阳性率的比较。结果ATP生物荧光法与金标大肠菌群快速检验纸片法相比,ATP生物荧光法阳性率为4.9%,纸片法阳性率为12%,ATP生物荧光检测法灵敏度为29%,特异性为98%,两者一致程度较差(k值为0.375)。结论ATP生物荧光法在食品卫生监督中受物品材质和环境因素影响较大,应根据周围环境、被采样品类型和采样部位进行合理安排。
Objective To explore the application of ATP bioluminescence assay in monitoring the hygiene of various catering utensils and hand sanitation of staff in cafeterias.Methods From January to June 2023,ATP bioluminescence assay was used to assess the cleanliness of 283 disinfected catering utensils and staff hands in a tertiary hospital cafeteria.The specificity,sensitivity,and positive rates of ATP bioluminescence assay were compared with the"gold standard method"(coliform rapid detection paper strip method).Results Compared to the coliform rapid detection paper strip method,the ATP bioluminescence assay showed a lower positive rate(4.9%vs 12%),lower sensitivity(29%),high specificity(98%),and poor agreement between the two methods(k value=0.375).Conclusion ATP bioluminescence assay is significantly influenced by material properties and environmental factors in food hygiene supervision.Its application should be tailored based on the surrounding environment,sampled specimen types,and sampling sites.
作者
崔朝丽
白云
常聪
何浩
蒋乃
贾恒川
CUI Chao-li;BAI Yun;CHANG Cong;HE Hao;JIANG Nai;JIA Heng-chuan(Disease Prevention and Control Department of Beyhune international peacehospital,Shijiazhuang,050000,China)
出处
《中国消毒学杂志》
2025年第5期373-375,共3页
Chinese Journal of Disinfection
关键词
ATP生物荧光检测法
纸片法
消毒
ATP bioluminescence assay
paper strip method
disinfection
