摘要
目的探讨红景天苷通过影响炎性反应发挥帕金森病(Parkinson’s disease,PD)的保护作用及机制探索。方法将小鼠分为五组,健康对照组,1-甲基-4-苯基-1,2,3,6-盐酸四氢吡啶(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine hydrochloride,MPTP)疾病组,红景天苷低剂量干预组、红景天苷中剂量干预组、红景天苷高剂量干预组。构建MPTP疾病小鼠,对MPTP小鼠进行红景天苷干预,通过行为学变化记录、炎性因子及自噬相关蛋白检测观察炎性反应、免疫组织化学染色观察神经元情况。结果MPTP处理后,小鼠出现显著的运动障碍,表现为运动迟缓、震颤及其他PD相关的行为学改变,促炎因子增多、抗炎因子减少,自噬相关蛋白降低,细胞焦亡明显;红景天苷干预可缓解以上变化,且随着给药浓度的增加,变化明显。结论在PD小鼠中,红景天苷可通过缓解炎性反应、促进自噬,发挥神经元的保护作用。
Aim To explore the neuroprotective effects of salidroside on Parkinson’s disease(PD)through modulation of inflammatory responses and the underlying mechanisms.Methods Mice were divided into five groups:healthy control group,1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)disease group,low-dose Rhodioloside intervention group,medium-dose salidroside intervention group,and high-dose salidroside intervention group.MPTP-induced PD mouse model was established,and salidroside intervention was administered.Behavioral changes,inflammatory cytokine levels,autophagy-related protein expression,and neurons were observed through histological analysis and immunohistochemical staining.Results After MPTP treatment,mice exhibited significant behavioral changes,increased pro-inflammatory cytokines,decreased anti-inflammatory cytokines,reduced autophagy-related proteins,and evident pyroptosis.Salidroside intervention alleviated these changes in a dose-dependent manner.Conclusions Salidroside exerts neuroprotective effects on PD by alleviating inflammatory responses and promoting autophagy,thereby protecting neurons.
作者
董小林
邬刚
李妍平
张丽娟
金富蓉
李锐
李红梅
张晓晓
李青芸
DONG Xiao-lin;WU Gang;LI Yan-ping;ZHANG Li-juan;JIN Fu-rong;LI Rui;LI Hong-mei;ZHANG Xiao-xiao;LI Qing-yun(Institute of Neurology,the Affiliated Yan’an Hospital of Kunming Medical University,Kunming 650051,China;Dept of Pharmacy,the Affiliated Yan’an Hospital of Kunming Medical University,Kunming 650051,China)
出处
《中国药理学通报》
北大核心
2025年第7期1340-1345,共6页
Chinese Pharmacological Bulletin
基金
云南省科技厅基础研究项目(No 202401AY070001-333)
云南省教育厅课题(No 2024J0284)
云南省心血管疾病重点实验室开发课题(No 2022SPR-01)。
关键词
红景天苷
帕金森病
运动障碍
炎性反应
自噬
细胞焦亡
salidroside
Parkinson’s disease
movement disorder
inflammatory response
autophagy
pyroptosis
