摘要
Effectively managing bone defects presents significant clinical challenges,where bone marrow mesenchymal stem cells(BMSCs)offer promising potential for bone regeneration.However,delivering BMSCs effectively requires suitable biomaterials.Herein,we introduce,Pep1,a calcium-responsive peptide designed for delivering BMSCs for femur regeneration.Pep1self-assembles to form a pericellular hydrogel in the presence of calcium ions,forming a nanofiber-rich network conductive to BMSCs encapsulation.Important,Pep1 hydrogel supports BMSC activity in 3D cell culture without compromise.In vitro and in vivo studies demonstrate excellent biocompatibility of Pep1 hydrogel.Osteogenic differentiation and q-PCR assays reveal that Pep1 enhances osteogenic differentiation and upregulates bone-related genes expression.Furthermore,In vivo experiments confirm that Pep1 hydrogel encapsulating BMSCs significantly improves femur defect repair.This study underscores Pep1 as a promising biomaterial for calcium-responsive bone tissue regeneration.
基金
supported by the National Natural Science Foundation of China(32401127)
the National Youth Talent Support Program(202309460011)
the Natural Science Foundation of Hunan(2024JJ5072)
the Science and Technology and Development Foundation of Shenzhen(JCYJ20210324122403010 and JCYJ20210324133611030)
the Medical and Health Technology Program of Zhejiang Province(2023KY148)。
