摘要
为探究红树林沉积物中细菌多样性及群落结构,通过高通量测序技术和传统培养方法,分析了沙港崀红树林沉积物的细菌多样性并预测其生态功能。结果表明,在6个采样点共检测到32个门、61个纲、207个属(包含未分类部分)的微生物类群,变形菌门、绿弯菌门、脱硫菌门、放线菌门和拟杆菌门为共同的优势菌门,γ-变形菌纲和厌氧绳菌纲为共同的优势菌纲,但不同样品之间细菌群落结构存在明显差异。FAPROTAX预测表明,与化能异养和光能自养有关的细菌类群的相对丰度较高。通过纯培养法共分离鉴定了310株细菌,分布于5门9纲23目50科106属,以ɑ-变形菌纲、γ-变形菌纲和芽孢杆菌纲为优势菌群,其中潜在新种属63株。本研究首次揭示了沙港崀红树林沉积物中的细菌群落组成,为红树林细菌资源的进一步开发以及潜在新物种的挖掘提供了科学依据。
In order to explore the diversity and distribution of the bacterial communities in the mangrove sediments,in the report,16S rRNA high-throughput sequencing and the traditional culture methods were used to analyze the microbial community characteristics of the sediment samples from Shaganglang mangrove and predict their ecological function.The results indicated that 32 phyla 61 classes and 207 genera(including the norank groups)of bacterial groups were detected,in which Proteobacteria,Chloroflexi,Desulfobacterota,Actinobacteriota and Bacteroidetes were the dominant bacteria,and the dominant microorganisms at class level were derived from Gammaproteobacteria and Anaerolineae.The prediction data from FAPROTAX suggested that the top 23 relative abundance metabolic functions of the bacterial communities mainly focused on the Chemoheterotrophy and Photoautotrophy.Based on the culture-dependent methods,a total of 310 bacteria strains being affiliated to 5 phyla,23 orders,50 families,and 106 genera were isolated and identified,in which Alphaproteobacteria,Gammaproteobacteria,and Bacilli were the dominant classes,and 63 strains were the potential new species.Our findings are helpful for understanding the diversity and distribution of bacterial communities in Shaganglang mangrove sediments,and which provide the basic data for further development of the bacterial resources as well as exploring potential new species.
作者
吴清娟
黄惠琴
许嘉芮
胡永华
莫坤联
WU Qingjuan;HUANG Huiqin;XU Jiarui;HU Yonghua;MO Kunlian(School of Marine Biology and Fisheries,Hainan University,Haikou 570228,China;Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China;Experimental Station in Zhanjiang,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang 524013,China)
基金
海南省自然科学基金青年基金项目(323QN278)
湛江市科技计划项目(2022A01050)。
