摘要
根据表面等离子体共振(surface plasma resonance, SPR)生物分子相互作用分析核心原理,提出了一种以折射率为理论基础并结合蔗糖标准物质的计量溯源方法。首先制备了蔗糖标准溶液,使用折光仪对其进行定值。然后将折射率值溯源至阿贝折射仪标准块,从而建立折射率的校准依据,实现折射率重复性检测、折射率标准值1.330~1.400范围内的线性校准,以及分析仪相对基线噪声和相对基线漂移的分析。最后,利用梯度稀释的蔗糖纯度标准物质,完成了检测限的校准。首次建立了全面、科学和有效的SPR生物分子相互作用分析的计量溯源方法,可应用于基础生命科学研究和生物医药研制领域的相关仪器计量校准应用。
According to the core principle of surface plasma resonance(SPR)biomolecular interaction analysis,a metrological traceability method was proposed,which takes the refractive index as the theoretical basis and combines sucrose reference materials.Firstly,sucrose standard solutions were prepared and quantified using a refractometer.Then,the refractive index values were traceable to the Abbe refractometer standard block,thereby establishing the calibration basis for the refractive index,realizing repeatability detection of the refractive index,linear calibration within the range of refractive index standard values from 1.330 to 1.400,and analysis of the analyzer’s relative baseline noise and relative baseline drift.Finally,using the purity reference materials of sucrose with gradient dilution,the calibration of the detection limit was completed.For the first time,a comprehensive,scientific,and effective metrological traceability method for SPR biomolecular interaction analysis has been established,which can be applied to the metrological calibration of related instruments in the fields of basic life science research and biopharmaceutical development.
作者
李兰英
陶晴
刘刚
LI Lanying;TAO Qing;LIU Gang(Key Laboratory of Bioanalysis and Metrology State Administration for Market Regulation,Shanghai Institute of Measurement and Testing Technology,Shanghai 201203,China)
出处
《计量学报》
北大核心
2025年第5期748-753,共6页
Acta Metrologica Sinica
基金
国家自然科学基金(22074093)
上海市市场监督管理局科技项目(2022-13)。
