摘要
为筛选出优异青稞种质,为青稞种质创新与新品种选育提供重要依据,采用变异系数、相关性、主成分及聚类分析方法,对西藏39份青稞种质资源进行遗传多样性分析和多维度系统评估。通过构建综合指标评价体系,实现了对样本遗传特征的定量化解析与分类研究。结果表明:39份青稞种质农艺性状变异系数范围为11.70%~68.80%,12个农艺性状间存在着不同的相关性,其中适当改良穗粒数、穗长、每穗小穗数等农艺性状可以相应提高青稞产量;主成分分析结果表明:青稞种质资源表型性状累计贡献率达到86.995%时,共提取出7个主成分;利用标准化后主成分特征值计算各主成分综合得分F值,获得排名前3的综合性状优异的青稞种质资源;39份青稞种质分为三大类群,第Ⅰ类群—第Ⅲ类群包括材料数分别为37、1、1份。对青稞种质材料进行多维度表型分析,结果显示供试材料在不同农艺性状指标间存在显著遗传变异。采用多元统计方法对青稞种质资源进行系统评估具有科学合理性。通过建立基于标准化主成分权重的综合评价模型,可实现对种质材料表型特征的定量化解析,为遗传改良提供可靠依据。进一步筛选出了Q9、Q3、Q34等性状优良的种质,可为青稞育种改良和市场推广提供选择;穗粒数、穗长、每穗小穗数可作为青稞种质评价的性状指标,用于西藏青稞农艺性状的遗传改良及种质资源的创新利用。
To analyze the genetic diversity of Highland barley germplasm resources in Xizang,screen out excellent barley germplasm,and provide an important basis for barley germplasm innovation and new variety breeding.This study used variation coefficient,correlation,prin-cipal component and cluster analysis methods to systematically evaluate 39 barley germplasm resources in Tibet in multiple dimensions.By constructing a comprehensive index evaluation system,the quantitative analysis and classification of the genetic characteristics of the samples were realized.The variation cofficient of 39 barley germplasm agronomic traits ranged from 11.70%to 68.80%,and there were different cor relations among 12 agronomic traits.Among them,the appropriate improvement of agronomic traits such as grains number per spike,spike length,and the spikelets number per spike could be increase yield of barley accordingly.The results of principal component analysis showed that the cumulative contribution rate of the phenotypic traits of barley germplasm reached 86.995%,and a total of 7 principal components were extracted.The comprehensive score F value of each principal component was calculated by using the standardized principal component ei-genvalues to obtain the top 3 barley germplasm resources with excellent comprehensive traits.Thirty nine samples of barley germplasm were divided into three major groups,the number of materials,included GroupI,II and II,were 37,1,1,respectively.In this study,a multidi-mensional phenotypic analysis of barley germplasm materials was carried out,and the results showed that there was significant genetic variation among different agronomic traits indicators of the test materials.It is scientifically reasonable to conduct the systematic evaluation of barley germplasm resources by using multivariate statistical methods.By establishing a comprehensive evaluation model based on standardized principal component weights,a quantitative analysis of the phenotypic characteristics of the germplasm materials can be realized for providing a reliable basis for genetic improvement.The gerrmplasm with excellent traits such as Q9,Q3 and Q34 were further screened out,which can provide choices for barley breeding improvement and market promotion.The grains number per spike,spike length,and spikelets number per spike can be used as trait indexes for the evaluation of highland barley germplasm,which can be used for the genetic improvement of agronom-ic traits of barley and the innovative utilization of germplasm resources in Xizang.
作者
董鑫
戴雨洋
强求旺姆
李梦寒
DONG Xin;DAI Yuyang;Qiang qiuwangmu;LI Menghan(Laboratory of Molecular Breeding of PlateauGrops,College of Plant Science,Xizang Agricultural and Animal Husbandry University,Nyingchi Xizang860000,China)
出处
《西藏农业科技》
2025年第2期13-21,共9页
Tibet Journal of Agricultural Sciences
基金
西藏农牧学院研究生创新计划资助项目--基于BSA-seq和RNA-seq的西藏玉米地方品种返祖性候选基因鉴定(YJS2025-05)。
关键词
青稞
种质资源
农艺性状
相关性分析
聚类分析
barley
germplasm resources
agronomic traits
correlation analysis
cluster analysis
