摘要
目的探讨己糖激酶2(HK2)在前列腺癌发生、发展过程中的生物学作用及其调控机制。方法采用免疫组织化学方法对良性前列腺增生组织和前列腺癌组织进行染色,以了解HK2的蛋白表达情况,并分析HK2与相关临床指标的关系;采用蛋白质印迹法探究HK2在各前列腺癌细胞系中的蛋白表达,并通过噻唑蓝(MTT)方法检测细胞活力;利用小鼠皮下成瘤模型观察肿瘤在动物体内的生长情况;采用同位素示踪的方法研究葡萄糖在前列腺癌细胞中的代谢流向。结果前列腺癌组织中HK2的表达高于良性前列腺增生组织(P<0.05)。HK2的表达强度与肿瘤的恶性程度、前列腺特异性抗原(PSA)水平以及有无远处转移呈正相关(P<0.05)。过表达HK2后,前列腺癌细胞增殖速率增快(P<0.01),敲低HK2后小鼠肿瘤发生率降低。HK2增强了前列腺肿瘤细胞中糖酵解通路的活性(P<0.05)。结论HK2通过调节糖酵解代谢通路的活性促进前列腺癌的发生、发展。
Objective To investigate the biological functions and regulatory mechanism of hexokinase(HK2)in driving tumor initiation and development of prostate cancer.Methods Immunohistochemical staining was employed to assess HK2 expression in benign prostate tissue and prostate cancer tissue.Correlation analyses were performed to elucidate the association of HK2 expression and clinical factors.Western blot analyses were employed to assess HK2 expression in prostate cancer cell lines.Cellular viability was assessed using the MTT assay.In vivo tumorigenesis ability of HK2 was evaluated using xenograft animal models.Glucose flux tracing was conducted through isotopic analyses in prostate cancer cells.Results HK2 expression was elevated in prostate cancer tissues compared to benign prostate tissues(P<0.05).HK2 expression was positively correlated with Gleason Score,prostate-specific antigen(PSA)levels and the presence of metastases(P<0.05).Overexpression of HK2 enhanced cell proliferation in vitro(P<0.01).Knockdown of HK2 decreased tumorigenesis in vivo.HK2 augmented metabolic activity of glycolysis pathway in prostate cancer cells(P<0.05).Conclusion HK2 facilitates tumorigenesis in prostate cancer by upregulating glycolytic activity.
作者
徐凌凡
丁和康
施浩强
杨诚
邰胜
Xu Lingfan;Ding Hekang;Shi Haoqiang;Yang Cheng;Tai Sheng(Dept of Urology,The First Affiliated Hospital of Anhui Medical University,Hefei 230022)
出处
《安徽医科大学学报》
北大核心
2025年第5期912-918,共7页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金项目(编号:82272886)
安徽省教育厅高校科研项目(编号:2022AH030118)
安徽省转化医学研究院科研基金项目(编号:2022zhyx-C37)。
关键词
前列腺癌
己糖激酶2
糖酵解
细胞增殖
免疫组化
同位素示踪
prostate cancer
hexokinase 2
glycolysis
cellular proliferation
immunohistochemical staining
isotopic tracing
