摘要
建立DB-FATWAX UI毛细管柱气相色谱仪法直接测定厌氧发酵液中挥发性脂肪酸和醇类检测方法。考虑是发酵液直接进入气相色谱仪进行检测,控制进样量为0.5μL,分流比10∶1,使用FID检测器,仪器程序升温进行快速测定样品中的醇类和挥发性脂肪酸。本研究确定了醇类、各类挥发性脂肪酸的保留时间、线性范围、准确度以及根据样品本身特性进行摸索一套仪器检测及维护流程。并在测试过程中,发现2.5 min保留时间出现一个新的未知物峰,经过实验验证获得该目标峰为新的产物正丙醇,截至目前,在餐厨垃圾厌氧发酵液中出现的正丙醇是新的碳源,一项新的发现,给予该行业新的发现价值。
A DB-FATWAX UI capillary column gas chromatograph method was developed for the direct determination of volatile fatty acids and alcohols in anaerobic fermentation broth of kitchen waste.It is considered that the fermentation liquid is directly entered into the gas chromatograph for detection.The injection volume is controlled to be 0.5μL,the splitter ratio is 10∶1,the FID detector is used,and the instrument program temperature is heated for rapid determination of alcohols and volatile fatty acids in the sample.This study determined the retention time,linear range and accuracy of alcohols and volatile fatty acids,and explored a set of instrument detection and maintenance procedures according to the characteristics of the sample itself.During the test,it was found that a new unknown peak appeared in the retention time of 2.5 min,and the target peak was verified by the test as a new product n-propanol.Up to now,the emergence of n-propanol in the anaerobic fermentation liquid of kitchen waste is a new product,which is a new discovery.
作者
陈银娣
伍青霞
伍汉坚
黄玉茂
冼威澄
刘勇劲
Chen Yindi;Wu Qingxia;Wu Hanjian;Huang Yumao;Xian Weicheng;Liu Yongjin(Guangdong Hanzheng Testing Technology Co.,Ltd.,Foshan 528225,China)
出处
《广东化工》
2025年第10期127-128,156,共3页
Guangdong Chemical Industry
关键词
气相色谱仪
色谱柱
色谱条件
仪器维护
正丙醇
gas chromatograph
chromatographic column
chromatographic condition
instrument maintenance
normal propyl alcohol
