摘要
为了对大豆ACC合成酶基因家族(ACO)进行筛选和表达分析,利用生物信息学筛选分析其基因序列、蛋白理化性质、启动子顺式元件、基因及蛋白结构及家族成员间进化关系;采用实时荧光定量PCR检测基因在根、茎和叶片中的表达模式。结果表明:大豆中筛选获得11条大豆ACOs同源序列,依据亚细胞定位预测结果发现大豆GmACOs基因家族编码的蛋白质分布于细胞质和细胞核中;对其与拟南芥和甜瓜等物种的ACO基因家族进行进化关系分析,结果表明大豆GmACOs基因家族进化关系与拟南芥和黄瓜更接近,所有GmACOs基因家族成员可分为3个组(GroupⅠ,GroupⅡ和GroupⅢ),其中GmACO3和GmACO4与AtACO5存在直系同源关系,GmACO7和GmACO8与AtACO1存在直系同源关系。GmACOs基因家族序列除发现GmACO_(2)基因序列不完整外,其他基因成员均含有2~3个内含子,这表明所有基因家族成员均具有非常高的保守性。基因表达分析结果表明,在根和叶中分别获得4条高盐胁迫响应基因,根中为GmACO1/3/4/7,叶中为GmACO1/2/8/11,可作为重要候选功能基因,表明大豆ACO基因家族成员响应盐胁迫的组织特异性存在一定差异,研究结果可为后续该基因家族成员参与大豆抗盐胁迫方面的深入研究提供理论基础。
In order to screen and analyze the ACC synthase gene family(ACO)in soybean,we used bioinformatics screening to analyze its gene sequence,protein physicochemical properties,promoter cis-elements,gene and protein structure,and evolutionary relationship among family members.Real-time PCR was used to detect the expression patterns of genes in roots,stems and leaves.The results showed that 11 homologous sequences of soybean ACOs were screened,and according to the results of subcellular localization prediction,the proteins encoded by the soybean GmACOs gene family were distributed in the cytoplasm and nucleus of the cells.The results showed that the evolutionary relationship of the soybean GmACOs gene family was closer to that of Arabidopsis thaliana and cucumber,and all GmACOs gene family members could be divided into three groups(GroupⅠ,GroupⅡand GroupⅢ),among which GmACO3 and GmACO4 were orthologous to AtACO5,and GmACO7 and GmACO8 were orthologous to AtACO1.Except for the incomplete GmACO_(2) gene sequence,the other gene members of the GmACOs gene family contained 2-3 introns,which indicated that all gene family members were highly conserved.The results of gene expression analysis showed that four genes responsive to high-salt stress were obtained in roots and leaves,GmACO1/3/4/7 in roots and GmACO1/2/8/11 in leaves respectively.GmACO7 was induced by high-salt stress in stems and could be used as an important candidate functional gene.This indicated that there were some differences in the tissue-specificity of soybean ACO gene-family members in response to salt stress.The results of this study could provide a theoretical basis for the subsequent in-depth study of the participation of soybean ACO gene-family members in soybean salt-stress resistance.
作者
张兴政
刘鑫
黄浩捷
孙一闻
董志遥
ZHANG Xingzheng;LIU Xin;HUANG Haojie;SUN Yiwen;DONG Zhiyao(College of Life Science/Key Laboratory of Plant Resource Science and Green Production,Jilin Normal University,Siping 136000,Jilin,China)
出处
《大豆科学》
北大核心
2025年第3期27-38,共12页
Soybean Science
基金
吉林省科技发展计划(自由探索一般项目,YDZJ202201ZYTS447)
国家自然科学基金(32101649)
吉林省教育厅科研基金(JJKH20230520KJ)。
