摘要
本研究结合雌二醇分子印迹技术、磁性二氧化硅和紫外可见光谱技术建立了一种快速测定雌二醇的方法。首先采用分子印迹技术对磁性二氧化硅复合材料进行改性,使其具有对雌二醇的特异性结合。结合后的复合材料经洗脱液洗脱,洗脱液在280 nm处具有最大吸收,最终实现雌二醇快速测定。以奶粉样品进行测试分析,方法检出限为0.25μg/kg,在较低浓度下的回收率可达99.6%。
A rapid determination of estradiol is established.Estradiol Molecular Imprinting Technology,silicon magnetic composite and UV-Visible spectrometry are well applied in this technique.Silicon magnetic composite is modified by Molecular Imprinting Technology,estradiol imprints on the layer successfully.The modified magnetic composite has a specific binding with estradiol.The complex is treated by eluent,the eluent is determinated at 280 nm by UV-Visible spectroscopy.Rapid determination of estradiol is realized ultimately.In the analyses of estradiol in milk powder,limit of detection in this research is 1.25μg/kg,recovery reaches 99.6%in lower concentration.
作者
许艳军
Xu Yan-jun(FuZhou Product Quatity Supervision and Inspection Institute,Fuzhou,FuJian 350008,China)
出处
《福建分析测试》
2025年第3期6-8,共3页
Fujian Analysis & Testing
关键词
分子印迹
雌二醇快速检测
Molecular Imprinting Technology
Rapid determination of estradiol
