摘要
植物碱性螺旋-环-螺旋(basic helix-loop-helix,bHLH)作为植物第二大类转录因子,广泛参与其生长发育、次生代谢合成调控等。本研究旨在全基因组范围内鉴定樟树(Cinnamomum camphora)bHLH转录因子,并研究其在两个化学型中的表达模式。本研究在全基因组范围内鉴定樟树bHLH家族成员,运用生物信息学分析其蛋白质理化性质、保守基序(motif)和系统进化等,基于樟树两个化学型(脑樟和芳樟)转录组数据分析bHLH成员的表达模式,并运用RT-qPCR进行验证。在樟树中鉴定出148个bHLH成员,大部分bHLH蛋白质分子富含酸性氨基酸且为亲水蛋白质;可分为24个亚家族,保守基序中motif 1和motif 2出现频率最高;转录组数据表明,27个bHLH基因在脑樟叶片中显著上调表达,分布于13个樟树bHLH亚家族,并用定量PCR验证了6个bHLH成员在两个化学型中的表达模式。本研究结果为进一步揭示樟树bHLH转录因子调控萜类物质的合成提供了参考依据。
Plant basic helix-loop-helix(bHLH)transcription factors are the second largest type of transcription factors in plants,and they play important roles in plant growth and development,regulation of secondary metabolism synthesis.The purpose of this study is to identify the genomic bHLH transcription factors,and the expression patterns of bHLHs between two chemical types of C.camphora.In this study,two chemical types of camphor-type and linalool-type C.camphora were sequenced with transcriptome,and bHLH gene family was identified,and bioinformatics methods were used to analyze their proteins physicochemical properties,conservative motifs,expression patterns,and phylogenetic analysis.The results showed that a total of 148 bHLHs were identified in C.camphora.Most of the bHLH proteins are rich in acidic amino acids and are hydrophilic.They can be classified into 24 subfamilies,with motif 1 and motif 2 being the most frequently observed in the conserved motifs.Transcriptome data revealed that 27 bHLH genes were significantly upregulated in C.camphora leaves and were distributed across 13 bHLH subfamilies.Furthermore,the expression patterns of six bHLH members in two chemotypes were validated by quantitative PCR(qPCR).These findings provide a reference for further elucidating the regulatory roles of bHLH transcription factors in terpenoid biosynthesis in C.camphora.
作者
周生财
林仕雄
宋敏燕
胡现铬
童再康
张俊红
Zhou Shengcai;Lin Shixiong;Song Minyan;Hu Xiange;Tong Zaikang;Zhang Junhong(College of Forestry Science and Technology,Lishui Vocational and Technical College,Lishui,323000;National Key Laboratory for Development and Utilization of Forest Food Resources,Zhejiang A&F University,Lin'an,311300)
出处
《分子植物育种》
北大核心
2025年第9期2948-2959,共12页
Molecular Plant Breeding
基金
浙江省农业(林木)新品种选育重大科技专项子课题(2021C02070-7-4)资助。
关键词
樟树
bHLH基因家族
萜类物质
化学型
Cinnamomum camphora
Basic helix-loop-helix(bHLH)gene family
Terpenoids
Chemical types
