摘要
IDD(INDETERMINATE DOMAIN)蛋白是一类特殊的植物转录因子,它广泛地参与植物的生长和逆境反应,然而IDD基因家族在葡萄中仍缺乏系统鉴定。本研究从公布的葡萄基因组数据库中,筛选出6个VvIDD基因,并用生物信息学技术对其基因的染色体定位、理化性质、结构功能、系统发育、表达特性等方面进行分析。结果表明:鉴定到分布在葡萄4条染色体上的6个Vv IDD家族基因,并命名为VvIDD1~VvIDD6。所有IDD蛋白均是亲水性的蛋白,没有跨膜结构和信号肽,在蛋白质的二级结构中α-螺旋和无规卷曲含量最多,三级结构既存在保守性又存在差异性。系统发育分析发现在VvIDD基因序列3'端均包含1个较长的外显子。通过构建拟南芥、水稻、苹果和葡萄的系统发育树,IDD基因家族成员可被划分为6个群体。顺式作用元件分析结果显示,VvIDD基因的上游启动子序列中含有大量光响应元件和激素响应元件,推测其与植物生长发育存在着一定的联系。通过对VvIDD基因的特异表达研究,发现大多数VvIDD基因在花后2和10周的表达水平较高,推测IDD基因可能在葡萄生长期、转色期起重要作用。本研究为深入了解葡萄Vv IDD基因家族的结构和功能提供理论依据。
The IDD(INDETERMINATE DOMAIN)family protein is a unique transcription factor of plants,which is widely involved in all stages of plant growth and adversity responses.However,the IDD gene family is still not systematically identified in grapes.In this study,six VvIDD genes were identified from the published grape genome database,and their chromosomal location,physical and chemical properties,structure and function,phylogeny and expression characteristics were analyzed by bioinformatics technology.The results showed that 6 VvIDD family genes distributed on 4 chromosomes of grapes were identified and named VvIDD1~VvIDD6.All IDD proteins are hydrophilic and do not contain signal peptides or transmembrane structures.The secondary structure of the protein contains the most random coil andα-helix,and the tertiary structure is both conserved and differentiated.Phylogenetic analysis revealed that all VvIDD gene sequences contain a long exon at the 3'end.The IDD gene family members can be divided into six groups by constructing phylogenetic trees for Arabidopsis,rice,apple and grape.Analysis of cis-acting elements demonstrated that the upstream promoter sequence of the VvIDD gene contains a large number of light-responsive and hormone-responsive elements,and it is speculated that members of this gene family may be closely related to plant growth and development.Gene specific expression analysis showed that most of the VvIDD genes were expressed in high abundance at 2 and 10 weeks after flowering in developing grapes,and it is speculated that IDD genes may play an important role in the growing period and veraison of grapes.This study provides a theoretical basis for further exploring the structure and function of the VvIDD gene family of grapes.
作者
赵士粤
马文培
黎霖
聂鸿飞
叶文秀
李桂荣
纪薇
Zhao Shiyue;Ma Wenpei;Li Lin;Nie Hongfei;Ye Wenxiu;Li Guirong;Ji Wei(Institute of Pomology,Shanxi Agricultural University,Taiyuan,030031;College of Horticulture,Shanxi Agricultural University,Taigu,030801;Yongji Municipal Agriculture and Rural Affairs Bureau,Yuncheng,044500;Institute of Modern Agriculture,Peking University,Weifang,261000;College of Horticulture and Landscape Architecture,Henan University of Science and Economics,Xinxiang,453003)
出处
《分子植物育种》
北大核心
2025年第8期2497-2510,共14页
Molecular Plant Breeding
基金
山西省现代农业产业技术体系建设专项(SXFRS-2022)
山西省高等学校科学研究优秀成果培育项目(2020-KJ037)
山西省研究生教育创新项目(2022Y335)
河南省科技攻关项目(222102110199)共同资助。
