摘要
在南四湖新薛河采集沉积物,利用富集培养基富集其中的微生物,初步培养并梯度稀释后,在分离培养基上继续培养,经初筛得到74株细菌。利用显色反应对其进行复筛,得到1株反硝化细菌L48。经过16S rRNA基因序列构建系统进化树,鉴定后发现L48与苏云金芽孢杆菌(Bacillus thuringiensis)处于同一分支,其亲缘关系最近。对其发酵培养条件进行了优化,得出最优发酵条件为接种量1.5%、初始pH值7.0、发酵时间24 h。在此条件下,反硝化细菌L48的活菌数目可达4.6×10^(14)CFU/mL。研究结果为沉积物中反硝化细菌的分离和筛选提供了方法参考,并为其在废水处理中的潜在应用提供了支持。
Sediments were collected from the Xinxue River of Nansi Lake.Microorganisms in the sediments were enriched using a selective medium,followed by preliminary cultivation and gradient dilution.Subsequent isolation on isolation media resulted in the identification of 74 bacterial strains.A colorimetric reaction-based secondary screening identified one denitrifying bacterium,designated L48.Phylogenetic analysis via 16S rRNA gene sequencing revealed that L48 clustered within the same clade as Bacillus thuringiensis,indicating a close genetic relationship.Fermentation conditions were optimized,with the optimal parameters determined as an inoculation volume of 1.5%,initial pH of 7.0,and fermentation duration of 24 hours.Under these conditions,the viable bacterial count of L48 reached 4.6×10^(14) CFU/mL.This study provided a methodological reference for isolating and screening denitrifying bacteria from sediments and supported their potential application in wastewater treatment.
作者
吴蒙蒙
孔勇
舒凤月
周晶
WU Mengmeng;KONG Yong;SHU Fengyue;ZHOU Jing(Shandong Freshwater Fisheries Research Institute,Jinan Shandong 250013;School of Life Sciences,Qufu Normal University,Qufu Shandong 273165)
出处
《现代农业科技》
2025年第11期127-131,共5页
Modern Agricultural Science and Technology
关键词
反硝化细菌
发酵条件
分离筛选
16S
rDNA测序
系统进化树
南四湖沉积物
denitrifying bacterium
fermentation condition
isolation and screening
16S rDNA sequencing
phylogenetic tree
sediment in Nansi Lake
