摘要
Brassica oleracea is an important biennial herbaceous species in the Cruciferae family.With an estimated 3.77 million hectares planted worldwide,these cole crops,for instance,cabbage,broccoli,and cauliflower,constitute significant agricultural resources(Li et al.,2024).In recent years,the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)technology has been extensively applied in major crops,including rice,wheat,and potato.This technology can be utilized to regulate or disrupt gene expression to develop high-quality,disease-resistant.
基金
supported by grants from the National Key R&D Program of China(2023YFE0111400)
the Key Technology R&D Program of Jiangsu Province(BE2023366)
S&T Program of Hebei Province(22322912D)
the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP-IVFCAAS)
the China Agriculture Research System of MOF and MARA(CARS-23)
Wulanchabu City's Chal enge and Recruitment Project(2022JB006)
partly supported by the Russian Ministry of Science and Higher Education(No.075-15-2023-582)。
