摘要
【目的】筛选并鉴定能够高效降低柠檬汁中有机酸质量浓度的微生物菌株,并评估其应用效果。【方法】通过微生物分离纯化技术筛选目标菌株,并优化其在柠檬汁降酸中的应用条件。【结果】经过筛选发现,酵母菌株Saturnispora diversa SD-59(SD-59)在高效降低有机酸的同时能产生令人舒适的风味物质。在最优培养条件(接种体积分数1.0%,温度31℃,转速200 r/min,培养时间60 h)下,柠檬汁的总酸质量浓度降低了97.9%,其中苹果酸和柠檬酸分别减少了100.0%和95.9%。【结论】SD-59具有高效的降酸能力,为生物降酸提供了新的菌种资源,有望在高酸环境中发挥重要作用。
[Objective]This study aims to screen and identify microbial strains capable of efficiently reducing the mass concentration of organic acids in lemon juice,and evaluate their application effects.[Method]Target strain was screened through microbial isolation and purification techniques,and the application conditions in the acidreducing process of lemon juice were optimized.[Result]After screening,it was found that the yeast strain Saturnispora diversa SD-59(SD-59)can efficiently reduce the content of organic acids while producing pleasant flavor compounds.Under the optimal culture conditions(inoculation volume fraction of 1.0%,temperature of 31℃,rotation speed of 200 r/min,culture time of 60 h),the total acid mass concentration in lemon juice was reduced by 97.9%,with malic acid and citric acid decreasing by 100.0%and 95.9%,respectively.[Conclusion]SD-59 exhibits remarkable acid-reducing capability,providing a novel microbial resource for biological deacidification and holding great promise for application in highly acidic environments.
作者
吴叶
陈星光
杨华
陆健
谢广发
吴殿辉
WU Ye;CHEN Xingguang;YANG Hua;LU Jian;XIE Guangfa;WU Dianhui(School of Biotechnology,Jiangnan University,Wuxi 214122,China;National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing,Jiangnan University,Wuxi 214122,China;Jiangsu Provincial Research Center for Bioactive Product Processing Technology,Jiangnan University,Wuxi 214122,China;Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province,Zhejiang Shuren University,Hangzhou 310015,China)
出处
《食品与生物技术学报》
北大核心
2025年第2期80-89,共10页
Journal of Food Science and Biotechnology
基金
安徽省科技重大专项(201903a06020030)
高等学校学科创新引智计划(111计划)项目(111-206)
江苏高校优势学科建设工程资助项目。
关键词
降酸
酵母
筛选
柠檬汁
acid decreasing
yeast
screening
lemon juice
