摘要
目的 对比分析两种类型保存袋4℃冷藏保存去白细胞混合浓缩血小板及22℃振荡保存去白细胞混合浓缩血小板的计数和功能变化差异。方法 普通血液保存袋4℃冷藏保存去白细胞混合浓缩血小板(A组)、血小板常温保存袋4℃冷藏保存去白细胞混合浓缩血小板(B组)于保存1、3、5、7、10、15、21 d,7个时间点与血小板常温保存袋22℃振荡保存去白细胞混合浓缩血小板(对照组)在保存1、3、5、7 d,4个时间点采集血样,采用血球计数仪、血栓弹力图仪、pH计、分光光度计对不同实验组的血小板进行检测。结果 A、B组血小板计数在保存期间均出现递减现象,但21 d时每袋血小板的总量大于2.5×10^(11);而对照组的血小板计数7 d变化不明显(P>0.05)。A、B组保存到15~21 d时,血栓弹力图指标(MA值、R值、K值、α角)和乳酸含量与对照组7 d时大致相当(P>0.05)。3组血小板pH值在保存期末均维持在7.0以上。结论 相较22℃振荡保存血小板,4℃静置冷藏保存血小板具有较好的聚集活性和较低的代谢率;采用普通血液保存袋或血小板常温保存袋4℃冷藏静置保存去白细胞混合浓缩血小板没有显著差异。
Objective To compare the platelet count and functional changes of leukocyte-depleted pooled platelet concentrates stored at 4℃in two different types of storage bags with those stored at 22℃under continuous agitation.Methods Leukocyte-depleted pooled platelet concentrates were divided into three groups:Group A,stored at 4℃in standard blood storage bags;Group B,stored at 4℃in platelet storage bags;and the Control Group,stored at 22℃with agitation in platelet storage bags.Samples were collected at days 1,3,5,7,10,15,and 21 for Groups A and B,and at days 1,3,5,and 7 for the Control Group.Platelet parameters were assessed using a hematology analyzer,thromboelastography(TEG),pH meter,and spectrophotometer.Results Both Groups A and B exhibited a gradual decline in platelet count over the storage period,yet the total platelet count per bag remained above 2.5×1011 even at day 21.In contrast,the Control Group maintained a relatively stable platelet count over 7 days(P>0.05).By days 15-21,thromboelastography parameters(MA,R,K,α-angle)and lactate levels in Groups A and B were comparable to those in the Control Group at day 7(P>0.05).The final pH values of all three groups remained above 7.0 at the end of their respective storage periods.Conclusion Compared to platelets stored at 22℃with agitation,platelets stored at 4℃under static conditions demonstrated superior aggregation activity and a lower metabolic rate.No significant differences were observed between standard blood storage bags and platelet storage bags when storing leukocyte-depleted pooled platelet concentrates at 4℃.
作者
曾四海
胡文娟
黄可君
刘静静
ZENG Si-hai;HU Wen-juan;HUANG Ke-jun;LIU Jing-jing(Blood Storage and dispensing Room,Guangzhou Blood Center,Guangzhou 510095,Guangdong,China)
出处
《广东医学》
2025年第4期559-564,共6页
Guangdong Medical Journal
基金
广州市科技计划项目(2003A03J1002)
广州市校(院)企联合资助专题(专项基础科研)项目(2060206-844)。
