摘要
目的 探讨LINC00152及LINC00265表达水平在儿童急性淋巴细胞白血病(acute lymphoblastic leukemia,ALL)血清中的表达及其临床意义。方法 前瞻性选取108例ALL患儿(ALL组)和50例健康儿童(对照组)纳入研究,根据ALL患儿诱导缓解化疗情况分为缓解组(n=65)和未缓解组(n=43)。采用实时荧光定量PCR技术检测血清LINC00152及LINC00265表达水平。绘制受试者工作特征(ROC)曲线分析LINC00152及LINC00265对儿童ALL的诊断价值。应用Kaplan-Meier法绘制生存曲线,采用多因素COX回归模型分析影响ALL患儿生存率的危险因素。结果 ALL组血清LINC00152(2.53±0.72 vs.1.20±0.26)、LINC00265(1.74±0.45 vs.0.85±0.13)表达水平均明显高于对照组(t=16.817、13.548,P均<0.001)。未缓解组血清LINC00152(2.95±0.84 vs.1.61±0.47)及LINC00265(1.92±0.53 vs.1.13±0.24)表达水平明显高于缓解组(t=16.905、11.326,P<0.001)。ROC曲线分析显示,LINC00152及LINC00265诊断儿童ALL的最佳截断值分别为1.94、1.31,其ROC曲线下面积分别为0.882(95%CI:0.819~0.943)、0.847(95%CI:0.791~0.912)。LINC00152和LINC00265高表达组淋巴结肿大、肝脾肿大、白细胞计数≥50×10^(9)·L^(-1)、血小板计数<100×10^(9)·L^(-1)、中高危险度分层、混合谱系白血病(MLL)重排及染色体核型异常的比例均明显高于LINC00152和LINC00265低表达组(P<0.05)。Kaplan-Meier分析显示,LINC00152和LINC00265高表达的ALL患儿生存期短(P<0.05)。多因素COX回归分析显示,肝脾肿大、中高危险度分层、MLL重排、LINC00152≥1.94及LINC00265≥1.31是影响ALL患儿生存率的危险因素。结论 LINC00152及LINC00265水平在ALL患儿血清中呈高表达,是影响ALL患儿生存率的危险因素,对指导临床诊断及预后判断具有一定意义。
Objective To investigate the expression levels of serum LINC00152 and LINC00265 in children with acute lymphoblastic leukemia(ALL)and their clinical significance.Methods A total of 108 children with ALL(ALL group)and 50 healthy children(control group)were enrolled prospectively.ALL patients were further divided into remission(n=65)and non-remission groups(n=43)based on the outcomes of induction remission chemotherapy.Serum levels of LINC00152 and LINC00265 were measured using real-time quantitative PCR.Receiver operating characteristic(ROC)curves were plotted to evaluate the diagnostic value of LINC00152 and LINC00265 for ALL.Kaplan-Meier survival curves were drawn,and a multivariate Cox regression model was used to analyze risk factors affecting survival.Results Serum levels of LINC00152(2.53±0.72 vs.1.20±0.26)and LINC00265(1.74±0.45 vs.0.85±0.13)were significantly higher in the ALL group than in the control group(t=16.817,13.548,P<0.001).Levels of LINC00152(2.95±0.84 vs.1.61±0.47)and LINC00265(1.92±0.53 vs.1.13±0.24)were significantly higher in the non-remission group than in the remission group(t=16.905,11.326,P<0.001).ROC curve analysis revealed optimal cut-off values of 1.94 for LINC00152(AUC=0.882,95%CI:0.819-0.943)and 1.31 for LINC00265(AUC=0.847,95%CI:0.791-0.912).Higher expression of LINC00152 and LINC00265 was associated with increased rates of lymphadenopathy,hepatosplenomegaly,WBC count≥50×109/L,platelet count<100×109/L,high-risk stratification,mixed lineage leukemia(MLL)rearrangement,and chromosomal abnormalities(P<0.05).Kaplan-Meier analysis indicated that high expression of LINC00152 and LINC00265 correlated with shorter survival(P<0.05).Multivariate Cox regression analysis identified hepatosplenomegaly,high-risk stratification,MLL rearrangement,LINC00152≥1.94,and LINC00265≥1.31 as independent risk factors affecting survival.Conclusion LINC00152 and LINC00265 are highly expressed in the serum of children with ALL and are associated with poor prognosis.Their levels have diagnostic and prognostic value,offering potential guidance for clinical decision-making.
作者
陈小翠
王莉雪
王文芳
陈秋丽
CHEN Xiao-cui;WANG Li-xue;WANG Wen-fang;CHEN Qiu-li(Department of Clinical Laboratory,Haikou Maternal and Child Health Hospital,Haikou 570100,Hainan,China;不详)
出处
《广东医学》
2025年第4期525-531,共7页
Guangdong Medical Journal
基金
海南省卫生计生行业科研项目(21A200074)。
