摘要
循环肿瘤细胞(CTCs)与肿瘤转移及患者预后密切相关。设计了一种多级筛选微流控芯片,旨在从癌症患者血液中高效分离、富集并培养CTCs,以支持精准治疗和动态监测。芯片利用CTCs与其他血液细胞在物理尺寸上的差异,设计了逐渐减小间隙的微柱结构,实现CTCs的高效分离和富集,分离后的CTCs通过密闭软管导入培养芯片,进行体外培养和检测。以5μm和10μm的微球以及4T1小鼠乳腺癌细胞混合液为模型,开展了分离、富集和培养实验。实验结果显示,在流速为0.5 mL/h的条件下,筛选芯片捕获率可达85%,经500μL/min流速注入培养芯片并在37℃培养24 h后,成功形成细胞系,便于后续观察细胞增殖与分化过程。本研究表明,该新型多级筛选微流控芯片在CTCs的分离、富集及体外培养方面具有显著的应用潜力,为肿瘤转移研究和个性化治疗提供了重要技术支持。
Circulating tumor cells(CTCs)are closely related to tumor metastasis and patient prognosis.In this study,a multi-stage screening microfluidic chip was designed to efficiently separate,enrich and culture CTCs from the blood of cancer patients to support precision treatment and dynamic monitoring.The chip uses the difference in physical size between CTCs and other blood cells to design a microcolumn structure with gradually reduced gaps to achieve efficient separation and enrichment of CTCs.The separated CTCs are introduced into the culture chip through a closed hose for in vitro culture and detection.In this study,5μm and 10μm microspheres and a mixture of 4T1 mouse breast cancer cells were used as models to carry out separation,enrichment and culture experiments.The experimental results showed that under the condition of a flow rate of 0.5 mL/h,the capture rate of the screening chip could reach 85%.After being injected into the culture chip at a flow rate of 500μL/min and cultured at 37℃for 24 hours,a cell line was successfully formed,which was convenient for subsequent observation of cell proliferation and differentiation processes.This study shows that this new multi-stage screening microfluidic chip has significant application potential in the separation,enrichment and in vitro culture of CTCs,providing important technical support for tumor metastasis research and personalized treatment.
作者
金家乐
宫平
JIN Jiale;GONG Ping(School of Life Science and Technology,Changchun University of Science and Technology,Changchun 130022)
出处
《长春理工大学学报(自然科学版)》
2025年第2期118-126,共9页
Journal of Changchun University of Science and Technology(Natural Science Edition)
基金
吉林省科技厅项目(20220204089YY,20240206006YY)。
关键词
循环肿瘤细胞
物理性质
微流控芯片
circulating tumor cells
physical property
microfluidic chip
