摘要
目的 分析Fe_(3)O_(4)@PDA标记的脐带间充质干细胞(hUCMSCs)在高糖(HG)环境下对肾小管上皮细胞(HK2细胞)的影响,探讨其对HG环境下HK2细胞自噬的作用机制。方法 合成Fe_(3)O_(4)@PDA纳米粒子(Fe_(3)O_(4)@PDA NPs),培养hUCMSCs、HK2细胞,将HK2细胞分为4组,包括(1)对照组(2)HG组(3)Fe_(3)O_(4)@PDA hUCMSCs组(4)hUCMSCs组。通过CCK8检测各组HK2细胞活力,Western blot检测各组HK2细胞中自噬相关蛋白Beclin1和p62的表达水平,RT-PCR检测各组HK2细胞自噬标志物(Beclin1、p62)mRNA的变化。结果 CCK8显示,HG组HK2细胞活力较空白对照组降低,与HG组对比,hUCMSCs组细胞活力显著升高(P<0.05),Fe_(3)O_(4)@PDA hUCMSCs组中HK2细胞活力升高更为明显(P<0.01);Western Blot和RT-PCR结果显示,HG组Beclin1蛋白和mRNA的表达较对照组减少,p62蛋白和mRNA的表达增加,与HG组相比,hUCMSCs组和Fe_(3)O_(4)@PDA hUCMSCs组的Beclin1蛋白和mRNA表达增加(P<0.05),p62蛋白和mRNA的表达减少(P<0.05),Fe_(3)O_(4)@PDA hUCMSCs组比hUCMSCs组变化更为显著。结论 Fe_(3)O_(4)@PDA标记的hUCMSCs可以通过诱导自噬减轻高糖对HK2细胞的损伤。
Objective To analyze the effect of Fe_(3)O_(4)@PDA-labeled umbilical cord mesenchymal stem cells(hUCMSCs)on renal tubular epithelial cells(HK2 cells)in a high glucose(HG)1 environment,and to explore the mechanism of autophagy in HK2 cells in HG environment.Methods Fe_(3)O_(4)@PDA nanoparticles(Fe_(3)O_(4)@PDA NPs)were synthesized,hUCMSCs and HK2 cells were cultured,and HK2 cells were divided into four groups,including control group@HG group@Fe_(3)O_(4) @PDA hUCMSCs group@hUCMSCs group.The viability of HK2 cells in each group was detected by CCK8,the expression levels of autophagy-related proteins Beclinl and p62 in HK2 cells from each group was detected by Western blot,and the changes of mRNA of autophagy markers(Beclinl,p62)in each group were detected by RT-PCR.Results CCK8 showed that HK2 cell viability was reduced in the HG group compared with the blank control group,and cell viability was significantly increased in the hUCMSCs group compared with the HG group(P<0.05),and HK2 cell viability was even more significantly increased in the Fe_(3)O_(4)@PDA hUCMSCs group(P<O.O1);the results of Western Blot and RT-PCR revealed that the HG group Beclinl protein and mRNA expression was decreased and p62 protein and mRNA expression was increased in the hUCMSCs group and Fe_(3)O_(4)@PDA hUCMSCs group compared with the control group(P<0.05),and Fe_(3)O_(4)@PDA hUCMSCs group had more significant changes than hUCMSCs group.Conclusion Fe O,@PDA labeled hUCMSCs could attenuate the damage of high glucose on HK2 cells by inducing autophagy.
作者
张金妹
高欢
司树涵
张晓羽
徐晓华
ZHANG Jinmei;GAO Huan;SI Shuhan;ZHANG Xiaoyu;XU Xiaohua(Department of Nephrology,China-Japan Union Hospital of Jilin University,Changchun 130033,China;Department of Nephrology,The Third People's Hospital of Jinan,Jinan 250000,China)
出处
《中国实验诊断学》
2025年第5期585-591,共7页
Chinese Journal of Laboratory Diagnosis
基金
吉林省科技发展计划项目(20210101294JC)。
