摘要
为阐明生物胺对草地螟Loxostege sticticalis幼虫密度依赖抗病性的调控作用,在室内温度为(22±1)℃,相对湿度为70%,光周期为L//D=16 h//8 h条件下,探究了不同密度:低密度(1头/瓶)、高密度(10头/瓶)、超高密度(30头/瓶)饲养的草地螟幼虫抗病能力和3种生物胺含量,以及外源生物胺对幼虫抗病免疫的影响。结果表明,不同密度幼虫的抗病能力差异显著,高密度(10头/瓶)幼虫对球孢白僵菌Beauveria bassiana的抵抗能力显著高于低密度(1头/瓶)和超高密度(30头/瓶)幼虫。高密度的幼虫血淋巴中章鱼胺含量明显高于其他2个密度。不同密度幼虫血淋巴中多巴胺和5-羟色胺的含量无显著变化。注射不同浓度(2、20、200μg/mL)外源章鱼胺后,幼虫被球孢白僵菌侵染后的存活率显著升高,对球孢白僵菌的抵抗能力明显增强。注射20μg/mL章鱼胺后,幼虫的酚氧化酶活性和血细胞包囊作用明显增强,血细胞总数显著增多,血细胞包囊率显著增加。以上结果表明,章鱼胺可调控草地螟幼虫的免疫功能以增强其抗病能力。因此,章鱼胺在草地螟幼虫密度依赖抗病免疫中具有重要作用。
To clarify the regulatory role of biogenic amines in density-dependent disease resistance in Loxostege sticticalis larvae,this study investigated disease resistance and biogenic amine levels of larvae reared at different larval densities:high density(10 larvae/jar),low density(1 larva/jar),and ultra-high density(30 larvae/jar)under controlled laboratory conditions[temperature:(22±1)℃,relative humidity:70%,photoperiod:L∥D=16 h∥8 h],and the effects of exogenous biogenic amines on the larval disease resistance and immunity.The results showed that disease resistance varied significantly across densities.High-density larvae(10 larvae/jar)exhibited significantly greater resistance to Beauveria bassiana than low-density larvae(1 larva/jar),while ultra-high-density larvae(30 larvae/jar)had significantly lower resistance.High-density larvae had significantly higher octopamine content than those at low-density and ultra-high-density.Dopamine and serotonin levels showed no significant differences among density groups.Exogenous octopamine injection enhanced disease resistance.Injection of 2,20,200μg/mL octopamine increased survival rates following B.bassiana infection.Injection of 20μg/mL octopamine resulted in increased phenoloxidase activity and hemocyte encapsulation,and a significant increase in total number of hemocytes and encapsulation rate.These results indicate that octopamine regulates the immune function of L.sticticalis larvae,enhancing disease resistance in high-density populations.Therefore,octopamine plays an important role in density-dependent disease resistance in L.sticticalis.
作者
陈志伟
程云霞
张蕾
徐林波
江幸福
孔海龙
CHEN Zhiwei;CHENG Yunxia;ZHANG Lei;XU Linbo;JIANG Xingfu;KONG Hailong(College of Plant Protection,Yangzhou University,Yangzhou 225009,China;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Institute of Grassland Research,Chinese Academy of Agricultural Sciences,Hohhot 010010,China)
出处
《植物保护》
北大核心
2025年第3期56-61,共6页
Plant Protection
基金
国家重点研发计划(2022YFD1400600)
国家自然科学基金(32172397)。
关键词
草地螟
幼虫密度
抗病能力
章鱼胺
免疫功能
Loxostege sticticalis
larval density
disease resistance
octopamine
immune funct
