摘要
目的探讨转录因子SOX2在胶质瘤中的表达及意义。方法采用反转录PCR(RTPCR)和Western blot方法分别检测胶质瘤细胞系U87、U251和C6细胞中SOX2 mRNA和蛋白的表达水平;采用免疫组化方法检测80例人脑胶质瘤组织和10例正常人脑组织中SOX2蛋白的表达,分析其表达水平与胶质瘤恶性程度和病理级别的关系。结果SOX2 mRNA和蛋白在U87、U251和C6细胞中均存在高表达。SOX2蛋白在人脑胶质瘤组织中高表达,而在正常人脑组织中几乎无表达,二者之间存在显著差异(r=0.503,P<0.01);SOX2蛋白表达在高恶性度胶质瘤中明显强于低恶性度胶质瘤(r=0.450,P<0.01),并且其表达随胶质瘤病理级别的升高而明显增强(r=0.379,P=0.012)。结论SOX2高表达与胶质瘤的病理学特征关系密切,其在胶质瘤的发生和发展过程中可能发挥重要作用。
Objective To investigate the expression status of transcription factor SOX2 and its significance in gliomas.Methods Reverse transcription PCR(RT-PCR)and Western blot were used to detect the expression levels of SOX2 mRNA and protein in glioma cell lines U87,U251 and C6,respectively.Immunohistochemical method was used to detect the expression of SOX2 protein in 80 human brain glioma tissues and 10 normal human brain tissues,and the relationship between its expression level and the malignant degrees and pathological grades of gliomas was analyzed.Results Both SOX2 mRNA and protein were highly expressed in U87,U251 and C6 cells.SOX2 protein was highly expressed in human brain glioma tissues,while it was almost not expressed in normal human brain tissues,and there was a significant difference between them(r=0.503,P<0.01);SOX2 protein expression in high-grade gliomas was significantly stronger than that in low-grade gliomas(r=0.450,P<0.01),and its expression also increased significantly with the increasing of glioma pathological grades(r=0.379,P=0.012).Conclusion SOX2 overexpression is closely related to the pathological characteristics of gliomas and may play an important role in the occurrence and development of gliomas.
作者
陈晓燕
霍军丽
郭勇
董秋峰
吕瑞
章翔
甄海宁
CHEN Xiaoyan;HUO Junli;GUO Yong;DONG Qiufeng;LYU Rui;ZHANG Xiang;ZHEN Haining(Department of Neurosurgery,Xijing Hospital,Air Force Medical University,Xi’an,Shaanxi 710032,China;State Key Laboratory of Cancer Biology,Air Force Medical University,Xi’an,Shaanxi 710032,China)
出处
《中华神经外科疾病研究杂志》
2025年第3期6-9,共4页
Chinese Journal of Neurosurgical Disease Research
基金
国家自然科学基金面上项目(81472358)
陕西省社会发展攻关计划项目(2014K11-01-02-07)
空军军医大学第一附属医院医务人员技术提升项目(2023XJSM05)
空军军医大学第一附属医院医务人员培养助推项目(XJZT24CY39)。
关键词
转录因子
SOX2
胶质瘤
表达
Transcription factor
SRY-related HMG box 2(SOX2)
Glioma
Expression
