摘要
目的:通过观察注意力缺陷多动(ADHD)大鼠在旷场实验、高架十字迷宫实验及水迷宫实验下的行为学表现,探讨四维止动方对ADHD大鼠行为学的影响。方法:将24只无特定病原体(SPF)级自发性高血压(SHR)大鼠随机分成SHR模型组、SHR中药组及SHR西药组,每组8只。引入8只SD大鼠作为正常组进行对照。在给药前后分别进行旷场实验、高架十字迷宫实验、水迷宫实验,比较各组及自身前后的数据结果。结果:SHR大鼠与SD大鼠比较,自发活动增多,烦躁,反应略迟钝。旷场实验中,给药前SHR模型组、SHR中药组、SHR西药组较正常组总路程增加、平均速度增快,自发活动增加(P<0.05)。给药后SHR中药组及SHR西药组均较给药前自发活动减少,但SHR中药组与正常组差异无统计学意义(P>0.05),SHR西药组自发活动少于正常组(P<0.05),西药组可能发生反向抑制作用。高架十字迷宫实验中各组给药前差异无统计学意义(P>0.05),给药后SHR模型组较给药前进入开放臂的时间及次数增加,其余组差异无统计学意义(P>0.05)。水迷宫实验中药组比模型组在给药后找到目标平台的总路程减少,平均速度增加,在目标区域停留的时间增加(P<0.05)。结论:四维止动方可以减少ADHD自发活动并无反向抑制,可以提高学习记忆能力。
Objective:To investigate the behavioral effects of Siwei Zhidong Formula on attention-deficit/hyperactivity disorder(ADHD) rats by observing their performance in the open field test,elevated plus maze test,and Morris water maze test.Methods:Twenty-four spontaneously hypertensive rats(SHR) of specific pathogen-free(SPF) grade were randomly divided into SHR model group,SHR Chinese medicine group,and SHR Western medicine group,with eight rats in each group.An additional eight Sprague-Dawley(SD) rats were included as a normal control group.Behavioral tests including the open field test,elevated plus maze test,and Morris water maze test were conducted before and after treatment to compare intergroup and intragroup results.Results:Compared with SD rats,SHR rats exhibited increased spontaneous activity,irritability,and slightly delayed responses.In the open field test before treatment,the SHR model group,SHR Chinese medicine group,and SHR Western medicine group showed increased total distance traveled,faster average speed,and increased spontaneous activity compared to the normal control group(P<0.05).After treatment,spontaneous activity in both the SHR Chinese medicine and SHR Western medicine groups decreased compared to that before treatment.The SHR Chinese medicine group showed no significant difference from the normal control group(P>0.05),while the SHR Western medicine group showed significantly lower spontaneous activity than the normal control group(P<0.05),suggesting a potential reverse inhibitory effect from the Western medicine.In the elevated plus maze test,there were no significant differences among groups before treatment(P>0.05).After treatment,the SHR model group showed an increase in the time spent and entries into the open arms,while other groups showed no significant changes(P>0.05).In the Morris water maze test,the SHR Chinese medicine group showed reduced total distance to reach the target platform,increased average swimming speed,and longer time spent in the target quadrant compared to the model group(P<0.05).Conclusion:Siwei Zhidong Formula can reduce spontaneous activity in ADHD rats without causing reverse inhibition and can enhance learning and memory abilities.
作者
魏巍
吴振起
王雪峰
赵雪
张睿
WEI Wei;WU Zhenqi;WANG Xuefeng;ZHAO Xue;ZHANG Rui(Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China;The Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110034,China;Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China)
出处
《世界中医药》
北大核心
2025年第6期944-949,共6页
World Chinese Medicine
基金
国家自然科学基金项目(81373687,81874490,82074494)
辽宁省沈阳市科学技术计划医工结合协同创新研究项目(223213401)
辽宁省自然科学基金计划项目(2022-YGJC-81)。
关键词
注意力缺陷多动
四维止动方
自发性高血压
大鼠
旷场实验
高架十字迷宫
水迷宫
行为学
中医经验方
Attention-deficit/hyperactivity disorder
Siwei Zhidong Formula
Spontaneously hypertensive
Rats
Open field test
Elevated plus maze
Morris water maze
Behavior
Chinese medicine empirical formula
