摘要
目的:研究黄连对雌性大鼠肠道菌群的影响,为临床合理使用黄连提供参考。方法:给予8周龄雌性大鼠灌胃不同剂量的黄连煎剂,于1、2、3周采集胃、十二指肠、空肠、回肠以及结肠(含肠内容物)标本,采用实时荧光定量PCR(qRT-PCR)技术,观察肠球菌、大肠杆菌、拟杆菌、乳酸菌及梭菌等5种肠道主要优势菌的结构和数量变化。结果:在十二指肠:黄连促进肠球菌的生长(P<0.05),先促进后抑制大肠杆菌、拟杆菌、乳酸菌以及梭菌的生长(P<0.05)。在空肠:黄连抑制肠球菌、大肠杆菌、拟杆菌、乳酸菌以及梭菌的生长(P<0.05)。在回肠:黄连促进肠球菌的生长,先促进后抑制大肠杆菌和乳酸菌的生长(P<0.05),抑制拟杆菌和梭菌的生长(P<0.05)。在结肠:黄连抑制肠球菌、大肠杆菌、拟杆菌、乳酸菌以及梭菌的生长(P<0.05)。在胃部:黄连促进大肠杆菌的生长(P<0.05),抑制肠球菌、乳酸菌的生长(P<0.05),先促进后抑制拟杆菌和梭菌的生长(P<0.05)。以上促进或抑制效应在不同的给药剂量、不同的连续给药时间及不同的肠段等条件下有着显著差异。结论:黄连对雌性大鼠的肠道菌群具有显著的调节作用,其影响程度在不同肠段中表现出明显差异,为进一步了解黄连的药理作用和机制提供新的思路和方法。
Objective:To investigate the effects of Rhizoma Coptidis(RC) on the intestinal microbiota of female rats for reference in the rational clinical use of RC.Methods:Eight-week-old female rats were administered with different doses of RC decoction by gavage.The specimens of duodenum,jejunum,ileum,and colon(including intestinal content) were collected at 1,2,and 3 weeks.The changes in the structure and quantity of five dominant intestinal microbiota,including Enterococcus,Escherichia coli,Bacteroides,Lactobacilli,and Clostridium,were observed by qRT-PCR technology.Results:In the duodenum,RC significantly promoted the growth of Enterococcus(P<0.05) while exhibiting a biphasic effect on Escherichia coli,Bacteroides,Lactobacilli,and Clostridium,initially promoting their growth,followed by inhibition(P<0.05).In the jejunum,RC demonstrated inhibitory effects on Enterococcus,Escherichia coli,Bacteroides,Lactobacilli,and Clostridium(P<0.05).In the ileum,RC promoted the growth of Enterococcus and showed a biphasic effect on Escherichia coli and Lactobacilli(P<0.05),while inhibiting the growth of Bacteroides and Clostridium(P<0.05).In the colon,RC inhibited the growth of Enterococcus,Escherichia coli,Bacteroides,Lactobacilli,and Clostridium(P<0.05).In the duodenum,RC promoted the growth of Escherichia coli(P<0.05),inhibited the growth of Enterococcus and Lactobacilli(P<0.05),and exhibited a biphasic effect on Bacteroides and Clostridium(P<0.05).Notably,these promoting or inhibitory effects varied significantly depending on different administration doses,continuous administration durations,and intestinal segments.Conclusion:RC has a significant regulatory effect on the intestinal microbiota of female rats,and the degree of its effect varies significantly among different intestinal segments.This study offers new ideas and methods to enhance the understanding of the pharmacological action and mechanism of RC.
作者
朱晨
袁丹丹
段学清
田维毅
ZHU Chen;YUAN Dandan;DUAN Xueqing;TIAN Weiyi(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)
出处
《世界中医药》
北大核心
2025年第6期915-923,共9页
World Chinese Medicine
基金
国家自然科学基金项目(82060824)
贵州省基础研究计划项目(黔科合基础-ZK[2022]一般512)
贵州省基础研究计划项目(黔科合基础-ZK[2022]一般460)。
关键词
黄连
雌性
5种肠道菌群
性别
肠段
剂量
影响
作用机制
Rhizoma Coptidis
Female
Five kinds of intestinal microbiota
Gender
Intestinal segment
Dose
Effect
Mechanism
