摘要
目的:分别应用中心组合设计-响应面法与正交试验,结合层次分析法(AHP)-熵权法(EWM)优化中药复方的提取工艺,为中药提取试验方法的优选提供依据。方法:以葛根素、橙皮苷、甘草苷转移率及复方出膏率的综合评分为响应值,以加水倍数、提取时间、提取次数为关键工艺参数,采用AHP-EWM法确定各种评价指标的权重系数,并进行综合评分,以中心组合响应面法及正交试验分别设计优化提取工艺参数,并进行验证与比较。结果:以参苓咀嚼片的提取工艺为例,分别采取正交试验法与响应面法得出的最佳工艺不同,但3批验证实验相对标准差(RSD)均小于3.0%,综合评分接近各自试验预测值。结论:响应面法工艺优选设计相对精细,但重复性和可操作性好,较正交试验更精细,更适合参苓咀嚼片的生产实际提取。
Objective:To optimize the extraction process of Chinese medicine compound formulas using both central composite design-response surface methodology(CCD-RSM) and orthogonal design,in combination with the analytic hierarchy process-entropy weight method(AHP-EWM),and to provide a reference for selecting optimal Chinese medicine extraction methodologies.Methods:The comprehensive score,based on the transfer rates of puerarin,hesperidin,and liquiritin,as well as the extract yield,was used as the response value.Water-to-material ratio,extraction time,and number of extractions were selected as key process parameters.The AHP-EWM was applied to determine the weight coefficients of each evaluation index and calculate the comprehensive score.Both CCD-RSM and orthogonal design were used to optimize the extraction parameters,followed by experimental validation and comparison.Results:Using the extraction process of Shenling Chewable Tablets as a case study,the optimal extraction conditions obtained by the orthogonal design and the response surface methodology were different.However,in both methods,the relative standard deviations(RSD) of three validation batches were all below 3.0%,and the comprehensive scores closely matched the predicted values from each respective design.Conclusion:The response surface methodology offers more refined process optimization with good reproducibility and operability,making it more suitable than the orthogonal design for practical production of Shenling Chewable Tablets.
作者
吴承潮
陆丽蓉
钟绍辉
鲁方奇
郑云枫
刘陶世
程建明
WU Chengchao;LU Lirong;ZHONG Shaohui;LU Fangqi;ZHENG Yunfeng;LIU Taoshi;CHENG Jianming(School of Pharmacy,Nanjing University of Chinese Medicine/Engineering Research Center of Classical Prescription,Nanjing 210023,China)
出处
《世界中医药》
北大核心
2025年第5期711-717,725,共8页
World Chinese Medicine
基金
国家自然科学基金项目(81973482)。
关键词
参苓咀嚼片
功能性食品
提取工艺优选
高效液相色谱法
层次分析法-信息熵权重法
中心组合-响应面法
正交试验法
生产实际
Shenling Chewable Tablets
Functional food
Optimization of extraction process
HPLC
Analytic hierarchy process-entropy weight method
Central composite design-response surface methodology
Orthogonal test
Practical production
