摘要
对桶状海绵共生真菌Penicillium rolfsii 8196#的次级代谢产物进行了研究,通过大米固体发酵培养、多种色谱技术分离纯化以及核磁共振、质谱等波谱数据分析,鉴定了9个化合物3,5-dichloro-2,6-dihydroxy-4-methyl-benzoic acid methyl ester(1)、methyl asterrate(2)、penicillither(3)、methyl dichloroasterrate(4)、2,4-dichloroasterric acid(5)、asterric acid(6)、5-hydroxymethylasterric acid(7)、sulochrin(8)、hydroxysulochrin(9),其中化合物1、2、7首次从青霉属真菌中分离得到。MTT法测试细胞毒活性,表明化合物6对T-24细胞有细胞毒性,IC_(50)值为16.60μmol/L;二倍稀释法抗菌活性测试显示,化合物4、5对沙门氏菌有弱抗菌活性,其MIC值均为50μmol/L;ABTS自由基清除实验中发现化合物8和9在浓度为10μmol/L时具有强抗氧化活性,自由基清除率分别达到了79.81%、70.84%。
Nine known aromatic compounds(1-9)were isolated from the ethyl acetate extracts of the solid cultures of marie-derived fungus Penicillium rolfsii 8196#,isolated from sponge xestospongia sp..Their structures were elucidated by spectroscopic analysis and comparative investigation with references.These compounds were evaluated for their cytotoxic,antibacterial and antioxidant activities.Among them,Compound 6 showed cytotoxicity against human bladder cancer T-24 cells with the IC_(50) values of 16.60μmol/L,compounds 4 and 5 exhibited weak inhibitory effects against Salmonella with MIC values of 50μmol/L,and compounds 8 and 9 showed the free radical scavenging rate 79.81%and 70.84%at the concentration of 10μmol/L,respectively.
作者
郑娜妹
刘洪亮
唐凤婷
李锦俊
张轩
杜志云
黄华容
刘岚
ZHENG Namei;LIU Hongliang;TANG Fengting;LI Jinjun;ZHANG Xuan;DU Zhiyun;HUANG Huarong;LIU Lan(School of Biomedicine,Guangdong University of Technology,Guangzhou 510006,China;School of Marine Science,Sun Yat-sen University,Zhuhai 519082,China)
基金
教育部省部共建药用资源化学与药物分子工程国家重点实验室开放基金(CMEMR2022-B03)。
关键词
海绵共附生真菌
青霉属
次级代谢产物
marine-derived fungus
Penicillium sp.
secondary metabolites
