摘要
目的探讨乳腺浸润性导管癌超声特征与上皮间质转化(EMT)基因表达之间的关系及临床意义。方法通过Pub Med数据库检索文献,使用美国国家生物技术信息中心和基于基因表达水平值的在线生信分析平台进行整合分析,选取相关EMT基因。收集2022年9月~2023年8月在山西医科大学第一医院就诊的38例经病理诊断为乳腺侵袭性导管癌的患者数据,包括临床表现及超声特征,探讨其与EMT基因表达之间的关系。结果共选取9个EMT基因,年龄在50岁以上或有淋巴结转移组患者VIM的表达水平高于其对照组(P<0.05)。EMT基因VIM、MMP2、ZEB1、ZEB2表达量与PI呈负相关关系(r=-0.500、-0.424、-0.548、-0.561,P<0.05),VIM、MMP2、ZEB1、ZEB2表达量与AUC呈负相关关系(r=-0.525、-0.470、-0.464、-0.522,P<0.05),ZEB1、ZEB2表达量分别与AT(r=0.329、0.403,P<0.05)、TTP(r=0.336、0.389,P<0.05)均呈正相关关系。与造影灌注均匀组相比,造影灌注不均匀组VIM、MMP2、MMP9和ZEB2的表达水平升高(P<0.05)。与灌注缺损面积≤1/2组相比,灌注缺损面积>1/2的VIM、MMP2、ZEB1和ZEB2水平升高(P<0.05)。在这些基因中,VIM、MMP2和ZEB2的表达明显相关。结论乳腺浸润性导管癌超声特征与EMT基因之间具有相关性,反映病灶内血管生成和缺氧坏死状态,可以为乳腺癌临床诊疗提供影像学依据,将EMT作为潜在的治疗靶点抑制肿瘤进展。
Objective To explore the relationship and clinical significance between ultrasound characteristics and the expression of epithelial mesenchymal transition(EMT)genes in breast invasive ductal carcinoma.Methods A literature search was conducted in the PubMed database,utilizing the National Center of Biotechnology Information and Gene Expression Profiting Interactive Analysis for comprehensive analysis to identify pertinent EMT genes.Gather information from 38 individuals identified with invasive ductal carcinoma of the breast through pathology at Shanxi Medical University's First Hospital from September 2022 to August 2023,encompassing clinical symptoms and ultrasound characteristics,to investigate their connection with EMT gene expression.Results Nine EMT genes were chosen in total.Notably,the expression level of VIM in patients over 50 years old or with lymph node metastasis was significantly higher than that in the control group(P<0.05).Additionally,the expression levels of the EMT genes VIM,MMP2,ZEB1,and ZEB2 were negatively correlated with PI(r=-0.500,-0.424,-0.548,-0.561,P<0.05),and similarly,these genes were also negatively correlated with AUC(r=-0.525,-0.470,-0.464,-0.522,P<0.05).Conversely,the expression levels of ZEB1 and ZEB2 exhibited positive correlations with AT(r=0.329,0.403,P<0.05)and TTP(r=0.336,0.389,P<0.05),respectively.Furthermore,compared to the uniform contrast perfusion group,the expression levels of VIM,MMP2,MMP9,and ZEB2 were elevated in the uneven contrast perfusion group.In addition,the levels of VIM,MMP2,ZEB1,and ZEB2 significantly increased in the group with a perfusion defect area greater>1/2,compared to the group with a perfusion defect area less≤1/2.Among these genes,VIM,MMP2,and ZEB2 showed significant correlations with one another.Conclusion A link exists between the ultrasound features of breast invasive ductal carcinoma and the EMT gene,offering imaging proof for clinical diagnosis and therapy,reflecting the angiogenesis and hypoxic necrosis status within the lesion,and EMT could be exploited as a potential therapeutic target to inhibit tumor progression.
作者
李欣榕
任家仪
胡道秀
曹静玮
陈耀东
陈武
LI Xinrong;REN Jiayi;HU Daoxiu;CAO Jingwei;CHEN Yaodong;CHEN Wu(College of Medical Imaging,Shanxi Medical University,Taiyuan 030001,China;Department of Ultrasound Imaging,the First Hospital of Shanxi Medical University,Taiyuan 030001,China)
出处
《分子影像学杂志》
2025年第5期562-568,共7页
Journal of Molecular Imaging
基金
山西省自然科学研究面上项目(20210302123259)。
关键词
乳腺癌
上皮间质转化
超声造影
血管生成
基因
breast cancer
epithelial mesenchymal transformation
contrast-enhanced ultrasound
angiogenesis
gene
