摘要
目的:研究CBX6对胶质瘤细胞侵袭的影响及分子机制。方法:构建稳定过表达CBX6胶质瘤细胞。transwell实验检测胶质瘤细胞侵袭性。qRT-PCR和蛋白质印迹法分别检测基因mRNA和蛋白表达水平。免疫组化检测人胶质瘤组织蛋白表达水平。Pearson相关分析胶质瘤组织基因表达量相关性。在过表达下游靶基因的情况下,transwell实验检测CBX6对胶质瘤细胞侵袭性的影响。结果:过表达CBX6显著降低胶质瘤U251MG细胞侵袭能力。机制研究发现CBX6抑制SNAI2表达。在胶质瘤组织中CBX6表达量和SNAI2表达量呈负相关,而且过表达SANI2可部分逆转CBX6对U251MG细胞侵袭的抑制作用。结论:CBX6可能通过沉默SNAI2表达抑制胶质瘤细胞侵袭。
Objective:To investigate the effects of CBX6 on the invasion of glioma cell and explore the potential molecular mechanisms.Methods:The stable overexpression of CBX6 in glioma cell was constructed.Glioma cell invasion was detected by transwell assay.mRNA and protein expression levels were detected by qRT-PCR and Western blot,respectively.Immunohistochemistry was used to detect the protein expression of human glioma tissues.Pearson correlation was used to analyze the correlation of gene expression level in glioma.In the case of overexpression of downstream target gene,the effect of CBX6 on the invasion of glioma cell was detected by transwell assay.Results:Overexpression of CBX6 significantly decreased the invasion ability of U251MG cell.Functional assays demonstrated that CBX6 could repress SNAI2 expression.There was a significantly negative correlation between the expression of CBX6 and SNAI2 in glioma tissues.Moreover,overexpression of SANI2 could partially reverse the inhibitory action of CBX6 on the invasion of U251MG cells.Conclusion:CBX6 inhibits invasion of glioma cell potential by silencing SNAI2 expression.
作者
邓后亮
莫小兰
DENG Houliang;MO Xiaolan(Department of Pharmacy,Guangzhou Women and Children’s Medical Center,Guangzhou Medical University,Guangzhou City,Guangdong Province 510120)
出处
《医学理论与实践》
2025年第11期1801-1804,1819,共5页
The Journal of Medical Theory and Practice
基金
国家自然科学基金项目(82103109)
广州市科技计划项目(2024A04J10002)。
