摘要
蛋白磷酸酶2C(PP2C)家族在植物响应逆境胁迫中发挥重要作用。本研究以中草28号老芒麦为材料,采用PCR技术成功克隆了EsPP2C48基因,并运用InterPro、NetPhos 3.1和STRING等生物信息学分析工具对EsPP2C48基因编码的蛋白质进行了结构和功能分析。结果显示,EsPP2C48是不含信号肽和跨膜区的亲水性蛋白,主要位于细胞质中,含有PPM型磷酸酶结构域。磷酸化位点预测表明,其有多个潜在的调控位点。EsPP2C48主要与丝氨酸/苏氨酸激酶中丝裂原活化蛋白激酶(MAPK)家族蛋白互作。以干旱胁迫0 h为对照,干旱胁迫下EsPP2C48基因在叶片中的表达量呈上调趋势,胁迫2 h时表达量显著高于对照(P<0.05),胁迫6 h时达到峰值,随后开始下降,胁迫12 h时仍显著高于对照(P<0.05),胁迫24 h和48 h时该基因的表达量仍高于对照,但差异不显著;干旱胁迫下,EsPP2C48在老芒麦根系中的表达量整体呈下调趋势,胁迫2 h、4 h时表达量小幅上调,但与对照没有显著差异,胁迫12 h后的表达量显著低于对照(P<0.05)。总而言之,EsPP2C48基因在老芒麦响应干旱胁迫过程中发挥着重要的调控作用。
The protein phosphatase 2C(PP2C)family plays an important role in plant responses to abiotic stress.In this study,the EsPP2C48 gene was successfully cloned from Elymus sibiricus cv.Zhongcao No.28 by PCR,using InterPro,NetPhos 3.1 and STRING bioinformatics analysis tools,the structure and function of the protein encoded by the EsPP2C48 gene were analyzed.The results indicate that EsPP2C48 was a hydrophilic protein without signal peptides and transmembrane domains,which was mainly located in the cytoplasm and contains a PPM-type phosphatase domain.Phosphorylation site prediction showed that there were multiple potential regulatory sites.EsPP2C48 mainly interacts with proteins of the mitogenactivated protein kinase(MAPK)family in serine/threonine kinases.Taking 0 hours of drought stress as the control,the expression level of EsPP2C48 gene in leaves showed an up-regulated trend under drought stress,which was significantly higher than that of the control at 2 h(P<0.05),reached the peak at 6 h,and then began to decline,the expression of this gene was significant higher than that of the control at 12 h(P<0.05),but not significantly higher than that of the control at 24 h and 48 h.The expression level of EsPP2C48 in the roots of Elymus sibircus was down-regulated as a whole,and up-regulated slightly after 2 h and 4 h of stress,but there was no significant difference compare with the control,after 12 h stress,the expression level was significantly lower than that of the control(P<0.05).In conclusion,EsPP2C48 gene plays an important regulatory role in the response of Elymus sibiricus to drought stress.
作者
李德珍
德英
闫伟红
杨忠
LI Dezhen;DE Ying;YAN Weihong;YANG Zhong(Institute of Grassland Research of CAAS,Hohhot 010010,China;Taipusi Banner Grassland Workstation,Xilingol 027000,China)
出处
《中国草地学报》
北大核心
2025年第5期46-54,共9页
Chinese Journal of Grassland
基金
国家自然科学基金项目(31802121)
内蒙古自治区科技计划项目(2020GG0063)。
