摘要
海参卵是海参加工的副产品,其富含多种生物活性成分,多糖是其中一种。为探究木瓜蛋白酶水解和黏质沙雷菌S2发酵对海参卵多糖结构和益生特性的影响,通过单独或联合利用木瓜蛋白酶水解和黏质沙雷菌发酵提取海参卵多糖。将所得多糖命名为SOP(未经木瓜蛋白酶水解、未经黏质沙雷菌S2发酵)、SOP-E(经木瓜蛋白酶水解、未经黏质沙雷菌S2发酵)、SOP-F(未经木瓜蛋白酶水解、经黏质沙雷菌S2发酵)和SOP-EF(木瓜蛋白酶水解结合黏质沙雷菌S2发酵)。结果表明,木瓜蛋白酶水解和黏质沙雷菌发酵可以影响海参卵多糖的提取产率、分子质量、单糖组成、微观结构和益生活性。结果显示:与其它提取方法相比,木瓜蛋白酶水解可使海参卵多糖的提取率提高约5倍,其中SOP-EF组分的分子质量最小(1.20×10^(5)u),与SOP和SOP-E相比,SOP-F的葡萄糖物质的量比下降而半乳糖含量升高,木瓜蛋白酶水解和黏质沙雷菌S2发酵均能有效降低海参卵多糖的粒径,SOP-E呈现由球形颗粒组成的疏松结构,而SOP-F则显示疏松多孔的网络结构。同时,海参卵多糖可作为益生元有效促进短双歧杆菌FB-1的生长。在24 h内,SOP和SOP-F显著增加了短双歧杆菌FB-1的数量(P<0.05);培养48 h后,SOP-F和SOP-EF相较于SOP-E和SOP组,更有效促进了短双歧杆菌FB-1的生长(P<0.05)。本研究证实木瓜蛋白酶水解与黏质沙雷菌S2发酵联合处理可作为获取具有优良益生活性海参卵多糖的有效途径。
Sea cucumber ovum is the by-product of the sea cucumber processing industry,and sea cucumber ovum polysaccharide is one of its active ingredients.To explore the effect of papain hydrolysis and Serratia marcescens fermentation on the structures and prebiotic properties of sea cucumber ovum polysaccharides,the polysaccharides were extracted through the sea cucumber ovum treated by papain hydrolysis and S.marcescens S2 fermentation individually or in combination.The obtained polysaccharides were designated as SOP(no papain hydrolysis and without S.marcescens S2 fermentation),SOP-E(papain hydrolysis but without S.marcescens S2 fermentation),SOP-F(no papain hydrolysis but fermented by S.marcescens S2),and SOP-EF(papain hydrolysis combined with S.marcescens S2 fermentation).The results revealed that papain hydrolysis and fermentation could influence the extraction yield,molecular weight,monosaccharide composition,microstructure,and prebiotic activities of the sea cucumber ovum polysaccharides.The results showed papain hydrolysis could improve the yield of the extracted polysaccharide by about 5-fold more than the other extraction methods.SOP-EF has the smallest molecular weight(1.20×10^(5) u).The glucose molar percentages of SOP-F declined while its galactose content increased.Papain hydrolysis and S.marcescens S2 fermentation decrease the particle size of the sea cucumber ovum polysaccharides;SOP-E exhibits a loose structure composed of sphere particles,while SOP-F exhibits a loose porous network structure.It was conceived that the sea cucumber ovum polysaccharides could be used as a prebiotic to promote the growth of Bifidobacterium breve FB-1.SOP and SOP-F significantly increased the bacteria number of B.breve FB-1 at 24 h than glucose(P<0.05);after incubation for 48 h,SOP-F and SOP-EF improved the growth of B.breve FB-1 more effectively than SOP-E and SOP(P<0.05).The results indicated that papain hydrolysis combined with Serratia marcescens S2 fermentation could be a meaningful way to acquire functional sea cucumber ovum polysaccharides with better prebiotic activities.
作者
檀茜倩
王晓晴
杨兆欣
崔方超
吕欣然
李英美
李学鹏
励建荣
TAN Xiqian;WANG Xiaoqing;YANG Zhaoxin;CUI Fangchao;LÜXinran;LI Yingmei;LI Xuepeng;LI Jianrong(College of Food Science and Engineering,Bohai University,Jinzhou 121013,Liaoning;Dalian Food Jinzhou Co.Ltd.,Jinzhou 121019,Liaoning)
出处
《中国食品学报》
北大核心
2025年第4期83-92,共10页
Journal of Chinese Institute Of Food Science and Technology
基金
辽宁省海洋经济发展专项(2021-84)。
关键词
海参卵
多糖
发酵
酶解
益生元
sea cucumber ovum
polysaccharide
fermentation
enzyme hydrolysis
prebiotic
