摘要
【目的】明确重庆市主要水稻产区的稻曲病菌[Ustilaginoidea virens(Cooke)Tak.]群体遗传多样性。【方法】对来源于重庆市铜梁(TL)、合川(HC)、永川(YC)、涪陵(FL)、万州(WZ)、梁平(LP)、忠县(ZX)、丰都(FD)、黔江(QJ)、秀山(XS)、彭水(PS)等11个区(县)的37株稻曲病菌进行重测序,并对其遗传多样性进行深入分析。【结果】对37个U.virens菌株重测序得到约42.5 Gb的高质量测序数据,其中检测到大量的单核苷酸突变。结合系统发育学和全基因组关系矩阵分析将37个菌株分为3个类群,表明不同群体的稻曲菌之间存在差异。所有群体除ZX以外,观测杂合度均大于期望杂合度,表明在重庆市稻曲菌群里面存在显著的杂合选择优势。PS群体拥有最高的Pi值,证明其样本间的差异程度最高,表明其遗传多样性最丰富。固定系数分析结果显示,绝大多数群体间的F_(ST)值>0.15,其中最高的F_(ST)值出现在HC和TL群体之间,为0.3814,表现出较高的遗传分化;而最低水平的遗传分化出现在YC和ZX群体之间,F_(ST)值为0.0537。群体结构分析发现所有菌株大致分为4个亚群。基因流(Nm)分析表明不同群体之间存在8次较大的基因交流,并且传播方向不是单一地从某个地区传入其他地区,而是存在不同区域间交叉、反复的传播事件。Tajima’s D中性检验结果显示,除LP以外,所有群体都表现出大于阈值的Tajima's D值(>0)。上述结果表明所有种群都在经历平衡选择。【结论】稻曲病菌在重庆的遗传多样性处于较高水平,为重庆地区稻曲病的防治和抗病资源选育提供理论依据。
[Objective]The paper aimed to explain the population genetic diversity of Ustilaginoidea virens(Cooke)Tak in major rice producing areas of Chongqing.[Method]37 strains of rice false smut from 11 districts in Chongqing,including Tongliang(TL),Hechuan(HC),Yongchuan(YC),Fulin(FL),Wanzhou(WZ),Liangping(LP),Zhongxian(ZX),Fengdu(FD),Qianjiang(QJ),Xiushan(XS)and Pengshui(PS),were selected for resequencing and subjected to an in-depth analysis of genetic diversity.[Result]A total of about 42.5 Gb of high-quality sequencing data was obtained by resequencing 37 isolates of U.virens,which contained a large number of single nucleotide polymorphism.Phylogenetic and genomic relationship matrix analysis divided the 37 samples into 3 groups,indicating differences between different groups of U.virens.In all populations except ZX,the observed heterozygosity was greater than the expected heterozygosity,indicating a significant heterozygote advantage within the U.virens populations in Chongqing.PS population had the highest Pi value,indicating the greatest degree of variation among its samples and reflecting the highest genetic diversity.The results of fixed coefficient analysis showed that the F_(ST)values of most populations were greater than 0.15,and the highest F_(ST)value was 0.3814 between HC and TL populations,indicating high genetic differentiation.The lowest level of genetic differentiation occurred between YC and ZX populations,with an F_(ST)value of 0.0537.The analysis of population structure showed that all strains were roughly divided into 4 subgroups.Gene flow(Nm)analysis showed that there were8 times significant substantial genetic exchanges between different populations,which revealed that the spread of U.virens was not a single transmission from one region to another,but there were mutual and repeated communication events among different regions.The results of Tajima's D neutrality test showed that Tajima's D values of(>0)all groups except LP were greater than the threshold.These results indicate that all populations were undergoing balanced selection.[Conclusion]The research results reveale that the population genetic diversity of U.virens in Chongqing is at a high level,providing a theoretical basis for the prevention and control of rice false smut and the selection of disease resistant resources in Chongqing.
作者
黎青
何焕然
李诗雨
郭静微
唐贵婷
魏真真
宋正富
LI Qing;HE Huan-ran;LI Shi-yu;GUO Jing-wei;TANG Gui-ting;WEI Zhen-zhen;SONG Zheng-fu(Chongqing Industry and Trade Polytechnic,Fuling,Chongqing 408000,China;Southeast Chongqing Academy of Agricultural Sciences,Fuling,Chongqing 408000,China;Sichuan Academy of Agricultural Sciences Institute of Plant Protection,Chengdu 610066,China)
出处
《西南农业学报》
北大核心
2025年第3期562-571,共10页
Southwest China Journal of Agricultural Sciences
基金
重庆市教育委员会科学技术研究项目(KJZD-K202103601)
重庆市教委科学技术研究项目(KJQN202203608)
重庆工贸职业技术学院科研项目(ZR202110)。
关键词
稻曲菌
全基因组重测序
遗传多样性
群体结构
Ustilaginoidea virens
Whole genome resequencing
Genetic diversity
Population structure
