摘要
为分析芍药不同部位代谢物的差异,以“奶油碗”品种芍药为研究对象,基于非靶向代谢组学技术,结合主成分分析、偏最小二乘法分析等方法,对花、叶、根中的代谢物进行差异性分析。结果共检测出1 654个代谢物,芍药的花和根中代谢物种类相似度较高,花和叶、花和根、叶和根中分别有511个、476个、490个差异代谢物,这些差异代谢物主要为脂质和类脂分子、有机酸及其衍生物以及苯丙烷和聚酮类化合物。代谢物主要分布于16条代谢途径中,富集程度最高的通路依次为全局和总览图、氨基酸代谢和其他次级代谢物的生物合成。本研究分析了芍药不同部位代谢物的差异性,为芍药资源的综合利用提供了理论依据。
To analyze the differences of metabolite in different parts of Paeonia lactiflora Pall.,the herbaceous peony cultivar named"Cream Bowl"was selected as material,based on non-targeted metabolomics technology combined with principal component analysis andpartial least squares analysis,the metabolites in the flowers,leaves and roots of herbaceous peony were explored.The results showed that 1654 metabolites were detected,the similarity of metabolite types in the flowers and roots of herbaceous peony is relatively high.There were 511,476,and 490 differential metabolites were found in flowers vs leaves,flowers vs roots,and leaves vs roots,respectively.These differential metabolites were mainly lipids and lipid molecules,organic acids and their derivatives,as well as phenylpropane and polyketide compounds.The metabolites were mainly distributed in 16 metabolic pathways,and the pathways with the highest enrichment of metabolites are global and overview maps,amino acid metabolism,and biosynthesis of other secondary metabolites.This study analyzed the differential metabolites in different parts of herbaceous peony,as well as provided theoretical basis for the comprehensive utilization of herbaceous peony resources.
作者
张璐哲
赵文双
孟兆青
崔莉
董晓弟
曹桂云
贺吉香
王岱杰
ZHANG Lu-zhe;ZHAO Wen-shuang;MENG Zhao-qing;CUI Li;DONG Xiao-di;CAO Gui-yun;HE Ji-xiang;WANG Dai-jie(School of Pharmaceutical Sciences,Shandong University of Traditional Chinese Medicine,Jinan250355,China;Qilu University of Technology(Shandong Academy of Sciences),Jinan250353,China;Shandong Shengshi-Shaohua Intelligent Agriculture Co.,Ltd.,Heze274035,China;Shandong Hongjitang Pharmaceutical Group Co.,Ltd.,Jinan 250100,China)
出处
《天然产物研究与开发》
北大核心
2025年第5期795-806,共12页
Natural Product Research and Development
基金
山东省重点研发计划(2023TZXD068,2024TSGC0998)
山东省重点研发计划重大科技创新工程项目(2021CXG C010511)
泉城5150引才倍增计划
关键词
芍药
不同部位
代谢组学
差异代谢物
Paeonia lactiflora Pall.
different parts
metabonomics
different metabolites
